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Deletion of NTH1 and HSP12 increases the freeze-thaw resistance of baker's yeast in bread dough.
Chen, Bo-Chou; Lin, Huan-Yu.
Afiliação
  • Chen BC; Bioresource Collection and Research Center, Food Industry Research and Development Institute, Hsinchu, 300, Taiwan.
  • Lin HY; Bioresource Collection and Research Center, Food Industry Research and Development Institute, Hsinchu, 300, Taiwan. hyl@firdi.org.tw.
Microb Cell Fact ; 21(1): 149, 2022 Jul 25.
Article em En | MEDLINE | ID: mdl-35879798
BACKGROUND: The intracellular molecule trehalose in Saccharomyces cerevisiae may have a major protective function under extreme environmental conditions. NTH1 is one gene which expresses trehalase to degrade trehalose. Small heat shock protein 12 (HSP12 expressed) plays a role in protecting membranes and enhancing freezing stress tolerance. RESULTS: An optimized S. cerevisiae CRISPR-Cpf1 genome-editing system was constructed. Multiplex genome editing using a single crRNA array was shown to be functional. NTH1 or/and HSP12 knockout in S. cerevisiae enhanced the freezing stress tolerance and improved the leavening ability after freezing and thawing. CONCLUSIONS: Deleting NTH1 in the combination with deleting HSP12 would strengthen the freezing tolerance and protect the cell viability from high rates of death in longer-term freezing. It provides valuable insights for breeding novel S. cerevisiae strains for the baking industry through a more precise, speedy, and economic genome-editing system.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Saccharomyces cerevisiae / Proteínas de Saccharomyces cerevisiae Idioma: En Ano de publicação: 2022 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Saccharomyces cerevisiae / Proteínas de Saccharomyces cerevisiae Idioma: En Ano de publicação: 2022 Tipo de documento: Article