A New Alternative Tool to Analyse Glycosylation in Monoclonal Antibodies Based on Drop-Coating Deposition Raman imaging: A Proof of Concept.

Hamla, Sabrina; Sacré, Pierre-Yves; Derenne, Allison; Cowper, Ben; Goormaghtigh, Erik; Hubert, Philippe; Ziemons, Eric

Hamla, Sabrina; Sacré, Pierre-Yves; Derenne, Allison; Cowper, Ben; Goormaghtigh, Erik; Hubert, Philippe; Ziemons, Eric.

Afiliação

Hamla S; Laboratory of Pharmaceutical Analytical Chemistry, Department of Pharmacy, University of Liege (ULiege), CIRM, Vibra-Sante Hub, 4000 Liege, Belgium.
Sacré PY; Laboratory of Pharmaceutical Analytical Chemistry, Department of Pharmacy, University of Liege (ULiege), CIRM, Vibra-Sante Hub, 4000 Liege, Belgium.
Derenne A; Center for Structural Biology and Bioinformatics, Laboratory for the Structure and Function of Biological Membranes, ULB, Campus Plaine CP206/02, 1050 Brussels, Belgium.
Cowper B; National Institute for Biological Standards and Control, Blanche Lane, South Mimms, Potters Bar, Hertfordshire EN6 3QG, UK.
Goormaghtigh E; Center for Structural Biology and Bioinformatics, Laboratory for the Structure and Function of Biological Membranes, ULB, Campus Plaine CP206/02, 1050 Brussels, Belgium.
Hubert P; Laboratory of Pharmaceutical Analytical Chemistry, Department of Pharmacy, University of Liege (ULiege), CIRM, Vibra-Sante Hub, 4000 Liege, Belgium.
Ziemons E; Laboratory of Pharmaceutical Analytical Chemistry, Department of Pharmacy, University of Liege (ULiege), CIRM, Vibra-Sante Hub, 4000 Liege, Belgium.

Molecules ; 27(14)2022 Jul 09.

Article em En | MEDLINE | ID: mdl-35889277

Assuntos

Antineoplásicos Imunológicos; Análise Espectral Raman; Anticorpos Monoclonais; Glicoproteínas; Glicosilação; Análise dos Mínimos Quadrados; Análise Multivariada; Análise Espectral Raman/métodos

Palavras-chave

P-vector; drop-coating deposition Raman imaging (DCDR); monoclonal antibodies (mAbs); multivariate curve resolution-alternating least square (MCR-ALS); nonlinear support vector regression (SVR); singular value decomposition (SVD)

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Análise Espectral Raman / Antineoplásicos Imunológicos Tipo de estudo: Prognostic_studies Idioma: En Ano de publicação: 2022 Tipo de documento: Article

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