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[Application value of metagenomic next-generation sequencing for pathogen detection in childhood agranulocytosis with fever]. / 病原体宏基因组二代测序在儿童粒细胞缺乏伴发热中的应用价值.
Zhu, Shan; Liu, Ying; Luo, Hai-Yan; Yang, Ming-Hua; Yang, Liang-Chun; Deng, Wen-Jun.
Afiliação
  • Yang LC; Department of Pediatrics, Xiangya Hospital of Central South University, Changsha 410008, China.
  • Deng WJ; Department of Pediatrics, Xiangya Hospital of Central South University, Changsha 410008, China.
Zhongguo Dang Dai Er Ke Za Zhi ; 24(7): 753-758, 2022 Jul 15.
Article em Zh | MEDLINE | ID: mdl-35894189
OBJECTIVES: To study the application value of metagenomic next-generation sequencing (mNGS) for pathogen detection in childhood agranulocytosis with fever. METHODS: A retrospective analysis was performed on the mNGS results of pathogen detection of 116 children with agranulocytosis with fever who were treated from January 2020 to December 2021. Among these children, 38 children with negative mNGS results were enrolled as the negative group, and 78 children with positive results were divided into a bacteria group (n=22), a fungal group (n=23), and a viral group (n=31). Clinical data were compared between groups. RESULTS: For the 116 children with agranulocytosis and fever, the median age was 8 years at diagnosis, the median turnaround time of mNGS results was 2 days, and the positive rate of mNGS testing was 67.2% (78/116). Compared with the negative group, the bacterial group had a higher procalcitonin level (P<0.05), the fungal group had higher level of C-reactive protein and positive rate of (1,3)-ß-D glucan test/galactomannan test (P<0.05), and the fungal group had a longer duration of fever (P<0.05). Among the 22 positive microbial culture specimens, 9 (41%) were consistent with the mNGS results. Among the 17 positive blood culture specimens, 8 (47%) were consistent with the mNGS results. Treatment was adjusted for 28 children (36%) with the mNGS results, among whom 26 were cured and discharged. CONCLUSIONS: The mNGS technique has a shorter turnaround time and a higher sensitivity for pathogen detection and can provide evidence for the pathogenic diagnosis of children with agranulocytosis and fever.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Agranulocitose / Metagenômica Tipo de estudo: Diagnostic_studies / Observational_studies Limite: Child / Humans Idioma: Zh Ano de publicação: 2022 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Agranulocitose / Metagenômica Tipo de estudo: Diagnostic_studies / Observational_studies Limite: Child / Humans Idioma: Zh Ano de publicação: 2022 Tipo de documento: Article