KOPI: Kinase inhibitOr Proteome Impact analysis.
Sci Rep
; 12(1): 13015, 2022 07 29.
Article
em En
| MEDLINE
| ID: mdl-35906361
ABSTRACT
Kinase inhibitors often exert on/off-target effects, and efficient data analysis is essential for assessing these effects on the proteome. We developed a workflow for rapidly performing such a proteomic assessment, termed as kinase inhibitor proteome impact analysis (KOPI). We demonstrate KOPI's utility with staurosporine (STS) on the leukemic K562 cell proteome. We identified systematically staurosporine's non-kinome interactors, and showed for the first time that it caused paradoxical hyper- and biphasic phosphorylation.
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1
Base de dados:
MEDLINE
Assunto principal:
Proteoma
/
Antineoplásicos
Idioma:
En
Ano de publicação:
2022
Tipo de documento:
Article