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CHIKV infection reprograms codon optimality to favor viral RNA translation by altering the tRNA epitranscriptome.
Jungfleisch, Jennifer; Böttcher, René; Talló-Parra, Marc; Pérez-Vilaró, Gemma; Merits, Andres; Novoa, Eva Maria; Díez, Juana.
Afiliação
  • Jungfleisch J; Molecular Virology group, Department of Medicine and Life Sciences, Universitat Pompeu Fabra, 08003, Barcelona, Spain.
  • Böttcher R; Molecular Virology group, Department of Medicine and Life Sciences, Universitat Pompeu Fabra, 08003, Barcelona, Spain. rene.boettcher@outlook.com.
  • Talló-Parra M; Molecular Virology group, Department of Medicine and Life Sciences, Universitat Pompeu Fabra, 08003, Barcelona, Spain.
  • Pérez-Vilaró G; Molecular Virology group, Department of Medicine and Life Sciences, Universitat Pompeu Fabra, 08003, Barcelona, Spain.
  • Merits A; Institute of Technology, University of Tartu, 50411, Tartu, Estonia.
  • Novoa EM; Centre for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology, 08003, Barcelona, Spain.
  • Díez J; Molecular Virology group, Department of Medicine and Life Sciences, Universitat Pompeu Fabra, 08003, Barcelona, Spain. juana.diez@upf.edu.
Nat Commun ; 13(1): 4725, 2022 08 11.
Article em En | MEDLINE | ID: mdl-35953468
ABSTRACT
Ample evidence indicates that codon usage bias regulates gene expression. How viruses, such as the emerging mosquito-borne Chikungunya virus (CHIKV), express their genomes at high levels despite an enrichment in rare codons remains a puzzling question. Using ribosome footprinting, we analyze translational changes that occur upon CHIKV infection. We show that CHIKV infection induces codon-specific reprogramming of the host translation machinery to favor the translation of viral RNA genomes over host mRNAs with an otherwise optimal codon usage. This reprogramming was mostly apparent at the endoplasmic reticulum, where CHIKV RNAs show high ribosome occupancy. Mechanistically, it involves CHIKV-induced overexpression of KIAA1456, an enzyme that modifies the wobble U34 position in the anticodon of tRNAs, which is required for proper decoding of codons that are highly enriched in CHIKV RNAs. Our findings demonstrate an unprecedented interplay of viruses with the host tRNA epitranscriptome to adapt the host translation machinery to viral production.
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Vírus Chikungunya / Febre de Chikungunya Limite: Animals / Humans Idioma: En Ano de publicação: 2022 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Vírus Chikungunya / Febre de Chikungunya Limite: Animals / Humans Idioma: En Ano de publicação: 2022 Tipo de documento: Article