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Molecular investigation of association between common IL-6 polymorphism with cytomegalovirus (CMV) infection and recurrent miscarriage in Iranian women.
Pourroostaei Ardakani, Parisa; Rahimi, Bahareh; Panahi, Mohammad; Sheykhi, Kazhaal; Karimian, Babak; Rahimi, Hamzeh.
Afiliação
  • Pourroostaei Ardakani P; Department of Molecular Medicine, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran.
  • Rahimi B; Department of Medical Biotechnology, Faculty of Allied Medical Sciences, Iran University of Medical Sciences, Tehran, Iran.
  • Panahi M; Department of Medical Biotechnology, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran.
  • Sheykhi K; Student Research Committee, Shahrekord University of Medical Sciences, Shahrekord, Iran.
  • Karimian B; Department of Molecular Medicine, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran.
  • Rahimi H; Texas Biomedical Research Center, San Antonio, TX, USA. rahimi.h1981@gmail.com.
Mol Biol Rep ; 49(10): 9499-9507, 2022 Oct.
Article em En | MEDLINE | ID: mdl-35962216
BACKGROUND: Recurrent pregnancy loss (RPL) is described as two or more spontaneous abortions. To date, scientists in various fields of knowledge, such as genetics, endocrinology, anatomy, immunology, and microbiology, have identified some important factors that affect abortions; nonetheless, the precise basic etiology is not determined in up to 50% of RPL cases. Human cytomegalovirus (CMV) infection and host genetic background, like IL-6 SNP polymorphisms, play important roles in RPL etiology. OBJECTIVE: This study aimed to evaluate the relationships among single nucleotide polymorphisms (-634C/G and -174 G/C) in the IL-6 gene with CMV infection and the risk of RPL for early detection and treatment. MATERIALS AND METHODS: This case-control study was carried on 80 Iranian females with RPL and 80 healthy females as controls. DNA was extracted from samples and CMV and IL6 SNPs were detected using Tetra ARMS-PCR. Statistics were analyzed by Epi Info TM and SPSS software by X2 test for the roles of CMV detection and two polymorphisms in RPL. RESULTS: The results indicated an increased rate of CMV infection in the RPL group (44%) compared to the control group (25.45%). The prevalence of IL-6-634C/G genotype among RPL patients with CMV infection was 80%, while the frequency of this genotype among RPL patients without CMV infection was 50%. Furthermore, no substantial relation was found between IL-6-174 G/C genotypes and RPL (p = 0.005). CONCLUSION: This study not only indicated a significant role for CMV in RPL, but also showed an association between CMV and allele G in IL6-634 among Iranian women. In addition, the findings suggested the use of CMV and IL-6-634 GG genotypes as diagnostic and prognostic biomarkers for RPL in the Iranian population.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Aborto Habitual / Infecções por Citomegalovirus Tipo de estudo: Observational_studies / Prognostic_studies / Screening_studies Limite: Female / Humans / Pregnancy País como assunto: Asia Idioma: En Ano de publicação: 2022 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Aborto Habitual / Infecções por Citomegalovirus Tipo de estudo: Observational_studies / Prognostic_studies / Screening_studies Limite: Female / Humans / Pregnancy País como assunto: Asia Idioma: En Ano de publicação: 2022 Tipo de documento: Article