MicroRNA-21-5p Regulates CD3+T Lymphocytes Through VCL and LTF in Patients with Immune Thrombocytopenia.
Clin Lab
; 68(7)2022 Jul 01.
Article
em En
| MEDLINE
| ID: mdl-35975534
ABSTRACT
BACKGROUND:
Immune thrombocytopenia (ITP) is an autoimmune hemorrhagic disease with distinct clinical manifestations such as extensive skin petechiae, mucosal bleeding, and even visceral hemorrhage. In this study, CD3+T lymphocytes from ITP patients were screened for differentially expressed genes. The expression of miR-21 and miR-155 in T lymphocytes of ITP patients were investigated. The downstream target genes of miR-21 and miR-155 were also searched for the correlation between differentially expressed genes of ITP.METHODS:
Differential gene screening was performed using the GSE43177 data set in the GEO database, and the expression of miR-21 and miR-155 in T lymphocytes of ITP patients was verified by qPCR. The interaction network of core downstream target genes and ITP differentially expressed genes of miR-21 and miR-155 were constructed with the STRING database, and the associated factors were verified by qPCR.RESULTS:
In ITP patients, the expression of CD8+T lymphocytes increased, the expression of CD4+T lymphocytes decreased, and the ratio of CD4+/CD8+T cells decreased. Fourteen genes were differentially expressed in CD3+T lymphocytes, all of which were upregulated, and the expression of S100A8 was increased in ITP patients. The expression of miR-21-5p and miR-155-5p increased in CD3+T lymphocytes of initial ITP patients. The core down-stream target gene VCL of miR-21 was associated with the differentially expressed genes such as LTF, LCN2, and DEFA4 in the interaction network. VCL expression was decreased and LTF expression was increased in ITP patients.CONCLUSIONS:
S100A8 plays an important role in the regulation of CD3+T lymphocytes in ITP patients. MiR-21-5p regulates the differentially expressed gene LTF by inhibiting the core downstream target gene VCL and participates in the immune mechanism of T lymphocytes in ITP patients. MiR-155-5p is also involved in the immunoregulatory mechanism of T lymphocytes in ITP patients.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Trombocitopenia
/
Púrpura Trombocitopênica Idiopática
/
MicroRNAs
Tipo de estudo:
Diagnostic_studies
Limite:
Humans
Idioma:
En
Ano de publicação:
2022
Tipo de documento:
Article