Your browser doesn't support javascript.
loading
Phosphorylation of RGS regulates MAP kinase localization and promotes completion of cytokinesis.
Simke, William C; Johnson, Cory P; Hart, Andrew J; Mayhue, Sari; Craig, P Lucas; Sojka, Savannah; Kelley, Joshua B.
Afiliação
  • Simke WC; Department of Molecular and Biomedical Sciences, University of Maine, Orono, ME, USA.
  • Johnson CP; Graduate School of Biomedical Science and Engineering, University of Maine, Orono, ME, USA.
  • Hart AJ; Department of Molecular and Biomedical Sciences, University of Maine, Orono, ME, USA.
  • Mayhue S; Department of Molecular and Biomedical Sciences, University of Maine, Orono, ME, USA.
  • Craig PL; Department of Molecular and Biomedical Sciences, University of Maine, Orono, ME, USA.
  • Sojka S; Department of Molecular and Biomedical Sciences, University of Maine, Orono, ME, USA.
  • Kelley JB; Department of Molecular and Biomedical Sciences, University of Maine, Orono, ME, USA joshua.b.kelley@maine.edu.
Life Sci Alliance ; 5(10)2022 10.
Article em En | MEDLINE | ID: mdl-35985794
Yeast use the G-protein-coupled receptor signaling pathway to detect and track the mating pheromone. The G-protein-coupled receptor pathway is inhibited by the regulator of G-protein signaling (RGS) Sst2 which induces Gα GTPase activity and inactivation of downstream signaling. G-protein signaling activates the MAPK Fus3, which phosphorylates the RGS; however, the role of this modification is unknown. We found that pheromone-induced RGS phosphorylation peaks early; the phospho-state of RGS controls its localization and influences MAPK spatial distribution. Surprisingly, phosphorylation of the RGS promotes completion of cytokinesis before pheromone-induced growth. Completion of cytokinesis in the presence of pheromone is promoted by the kelch-repeat protein, Kel1 and antagonized by the formin Bni1. We found that RGS complexes with Kel1 and prefers the unphosphorylatable RGS mutant. We also found overexpression of unphosphorylatable RGS exacerbates cytokinetic defects, whereas they are rescued by overexpression of Kel1. These data lead us to a model where Kel1 promotes completion of cytokinesis before pheromone-induced polarity but is inhibited by unphosphorylated RGS binding.
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Proteínas Quinases Ativadas por Mitógeno / Proteínas RGS / Proteínas de Saccharomyces cerevisiae / Citocinese Idioma: En Ano de publicação: 2022 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Proteínas Quinases Ativadas por Mitógeno / Proteínas RGS / Proteínas de Saccharomyces cerevisiae / Citocinese Idioma: En Ano de publicação: 2022 Tipo de documento: Article