Protocol for generation of 3D bone marrow surrogate microenvironments in a rotary cell culture system.
STAR Protoc
; 3(3): 101601, 2022 09 16.
Article
em En
| MEDLINE
| ID: mdl-35990738
ABSTRACT
In this protocol, we describe how to generate 3D culture surrogates of chronic lymphocytic leukemia (CLL) and multiple myeloma (MM) bone marrow microenvironments. We detail the use of culturing scaffolds populated with BM stromal cells and tumor cells in the RCCS™ bioreactor. This 3D culture can efficiently recapitulate tumor-stroma crosstalk and allows the testing of drugs such as ibrutinib and bortezomib. Moreover, this protocol can be used for the generation of other and more complex tumor microenvironments. For complete details on the use and execution of this protocol, please refer to Belloni et al. (2018) and Barbaglio et al. (2021).
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Base de dados:
MEDLINE
Assunto principal:
Medula Óssea
/
Mieloma Múltiplo
Limite:
Humans
Idioma:
En
Ano de publicação:
2022
Tipo de documento:
Article