A Proteomic Approach for the Quantification of Posttranslational Protein Lysine Acetylation in Candida albicans.
Methods Mol Biol
; 2542: 41-54, 2022.
Article
em En
| MEDLINE
| ID: mdl-36008655
ABSTRACT
Candida albicans is a normal component of the human microflora that colonizes mucosal/epithelial surfaces and the gastrointestinal tract as a commensal organism. However, in an immunocompromised host, it can cause life-threatening infections of high mortality and morbidity. Virulence as well as antifungal drug resistance of C. albicans is often regulated by posttranslational modifications (PTM) of proteins via lysine acetylation by lysine acetyltransferases. Here, we report an experimental approach using tandem mass tag (TMT) labeling for the detection and quantification of lysine acetylation of histone and nonhistone proteins in C. albicans.
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Base de dados:
MEDLINE
Assunto principal:
Candida albicans
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Lisina
Limite:
Humans
Idioma:
En
Ano de publicação:
2022
Tipo de documento:
Article