Tri-mode optical biopsy probe with fluorescence endomicroscopy, Raman spectroscopy, and time-resolved fluorescence spectroscopy.
J Biophotonics
; 16(2): e202200141, 2023 02.
Article
em En
| MEDLINE
| ID: mdl-36062395
ABSTRACT
We present an endoscopic probe that combines three distinct optical fibre technologies including A high-resolution imaging fibre for optical endomicroscopy, a multimode fibre for time-resolved fluorescence spectroscopy, and a hollow-core fibre with multimode signal collection cores for Raman spectroscopy. The three fibers are all enclosed within a 1.2 mm diameter clinical grade catheter with a 1.4 mm end cap. To demonstrate the probe's flexibility we provide data acquired with it in loops of radii down to 2 cm. We then use the probe in an anatomically accurate model of adult human airways, showing that it can be navigated to any part of the distal lung using a commercial bronchoscope. Finally, we present data acquired from fresh ex vivo human lung tissue. Our experiments show that this minimally invasive probe can deliver real-time optical biopsies from within the distal lung - simultaneously acquiring co-located high-resolution endomicroscopy and biochemical spectra.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Análise Espectral Raman
/
Endoscopia
Limite:
Humans
Idioma:
En
Ano de publicação:
2023
Tipo de documento:
Article