De novo assembly and annotation of the CHOZN® GS-/- genome supports high-throughput genome-scale screening.
Biotechnol Bioeng
; 119(12): 3632-3646, 2022 Dec.
Article
em En
| MEDLINE
| ID: mdl-36073082
ABSTRACT
Chinese hamster ovary (CHO) cells have been used as the industry standard for the production of therapeutic monoclonal antibodies for several decades. Despite significant improvements in commercial-scale production processes and media, the CHO cell has remained largely unchanged. Due to the cost and complexity of whole-genome sequencing and gene-editing it has been difficult to obtain the tools necessary to improve the CHO cell line. With the advent of next-generation sequencing and the discovery of the CRISPR/Cas9 system it has become more cost effective to sequence and manipulate the CHO genome. Here, we provide a comprehensive de novo assembly and annotation of the CHO-K1 based CHOZN® GS-/- genome. Using this platform, we designed, built, and confirmed the functionality of a whole genome CRISPR guide RNA library that will allow the bioprocessing community to design a more robust CHO cell line leading to the production of life saving medications in a more cost-effective manner.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Genoma
/
Sistemas CRISPR-Cas
Tipo de estudo:
Diagnostic_studies
/
Screening_studies
Limite:
Animals
Idioma:
En
Ano de publicação:
2022
Tipo de documento:
Article