Synthesis of ginsenoside Rb<sub>1</sub>-imprinted magnetic polymer nanoparticles for the extraction and cellular delivery of therapeutic ginsenosides.

Synthesis of ginsenoside Rb₁-imprinted magnetic polymer nanoparticles for the extraction and cellular delivery of therapeutic ginsenosides.

Liu, Kai-Hsi; Lin, Hung-Yin; Thomas, James L; Shih, Yuan-Pin; Yang, Zhuan-Yi; Chen, Jen-Tsung; Lee, Mei-Hwa.

Afiliação

Liu KH; Department of Internal Medicine, Division of Cardiology, Zuoying Branch of Kaohsiung Armed Forces General Hospital, Kaohsiung, Taiwan.
Lin HY; Department of Electrical Engineering, National University of Kaohsiung, Kaohsiung, Taiwan.
Thomas JL; Department of Chemical and Materials Engineering, National University of Kaohsiung, Kaohsiung, Taiwan.
Shih YP; Department of Physics and Astronomy, University of New Mexico, Albuquerque, NM, USA.
Yang ZY; Department of Chemical and Materials Engineering, National University of Kaohsiung, Kaohsiung, Taiwan.
Chen JT; Department of Chemical Engineering, I-Shou University, Kaohsiung, Taiwan.
Lee MH; Department of Life Sciences, National University of Kaohsiung, Kaohsiung, Taiwan.

J Ginseng Res ; 46(5): 621-627, 2022 Sep.

Article em En | MEDLINE | ID: mdl-36090682

ABSTRACT

Background:

Panax ginseng (ginseng) is a traditional medicine that is reported to have cardioprotective effects; ginsenosides are the major bioactive compounds in the ginseng root.

Magnetic molecularly imprinted polymer (MMIP) nanoparticles might be useful for both the extraction of the targeted (imprinted) molecules, and for the delivery of those molecules to cells. In this work, plant growth regulators were used to enhance the adventitious rooting of ginseng root callus; imprinted polymeric particles were synthesized for the extraction of ginsenoside Rb1 from root extracts, and then employed for subsequent particle-mediated delivery to cardiomyocytes to mitigate hypoxia/reoxygenation injury.

These synthesized composite nanoparticles were first characterized by their specific surface area, adsorption capacity, and magnetization, and then used for the extraction of ginsenoside Rb1 from a crude extract of ginseng roots. The ginsenoside-loaded MMIPs were then shown to have protective effects on mitochondrial membrane potential and cellular viability for H9c2 cells treated with CoCl2 to mimic hypoxia injury. The protective effect of the ginsenosides was assessed by staining with JC-1 dye to monitor the mitochondrial membrane potential.