Inhibitory mechanism of ternary fusion protein MSIK on HPV virus.

ABSTRACT

OBJECTIVE: We investigated whether the ternary fusion protein (MBP-SERPINA3-IFN-κ, MSIK) can inhibit the proliferation of human papillomavirus (HPV) and the possible mechanism of this inhibitory effect. MATERIALS AND METHODS: First, the purchased MSIK protein was prepared into MSIK protein solutions of different concentrations, and then epithelial cells and 300 mice were involved in the research. The HSV-1 virus was used as the infection pathogen to explore the mechanism of MSIK reinhibition of HPV virus. The virus content in each sample was detected by PCR technology. RESULTS: Epithelial cells treated with different concentrations of MSIK had inhibitory effects on the invasion and replication of HSV-1 virus. MSIK protein had a positive effect on wound healing in mice at the same time, and it had an inhibitory effect on the invasion of HSV-1 virus, and the higher concentration of MSIK, the better the effect. CONCLUSIONS: MSIK can quickly help wound healing and inhibit the replication of HSV-1 virus. It can also effectively prevent the influx of HPV virus and replication has a positive effect on the prevention and treatment of HPV virus.