HPLC fluorescence assay for measuring the activity of diacylglycerol lipases and the action of inhibitors thereof.

Depmeier, Tim; Lange, Thomas; Hanekamp, Walburga; Strünker, Timo; Lehr, Matthias

Depmeier, Tim; Lange, Thomas; Hanekamp, Walburga; Strünker, Timo; Lehr, Matthias.

Afiliação

Depmeier T; Institute of Pharmaceutical and Medicinal Chemistry, University of Münster, Corrensstrasse 48, 48149, Münster, Germany.
Lange T; Institute of Pharmaceutical and Medicinal Chemistry, University of Münster, Corrensstrasse 48, 48149, Münster, Germany.
Hanekamp W; Institute of Pharmaceutical and Medicinal Chemistry, University of Münster, Corrensstrasse 48, 48149, Münster, Germany.
Strünker T; Centre of Reproductive Medicine and Andrology, University Hospital Münster, University of Münster, Domagkstrasse 11, 48149 Münster, Germany.
Lehr M; Institute of Pharmaceutical and Medicinal Chemistry, University of Münster, Corrensstrasse 48, 48149, Münster, Germany. Electronic address: lehrm@uni-muenster.de.

Anal Biochem ; 657: 114889, 2022 11 15.

Article em En | MEDLINE | ID: mdl-36113549

Assuntos

Endocanabinoides; Lipase Lipoproteica; Animais; Cromatografia Líquida de Alta Pressão; Diglicerídeos; Glicerídeos; Humanos; Masculino; Pirenos; Ratos; Sêmen

Palavras-chave

Diacylglycerol lipase; Fluorescence assay; HPLC; Inhibitor; Pyrene-labeled substrate

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Endocanabinoides / Lipase Lipoproteica Limite: Animals / Humans / Male Idioma: En Ano de publicação: 2022 Tipo de documento: Article

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