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Metabolic engineering of Mannheimia succiniciproducens for malic acid production using dimethylsulfoxide as an electron acceptor.
Lee, Jong An; Ahn, Jung Ho; Kim, Gi Bae; Choi, Sol; Kim, Ji Yeon; Lee, Sang Yup.
Afiliação
  • Lee JA; Department of Chemical and Biomolecular Engineering (BK21 Four Program), Metabolic and Biomolecular Engineering National Research Laboratory, Systems Metabolic Engineering and Systems Healthcare Cross-Generation Collaborative Laboratory, Institute for the BioCentury, Korea Advanced Institute of Scie
  • Ahn JH; Department of Chemical and Biomolecular Engineering (BK21 Four Program), Metabolic and Biomolecular Engineering National Research Laboratory, Systems Metabolic Engineering and Systems Healthcare Cross-Generation Collaborative Laboratory, Institute for the BioCentury, Korea Advanced Institute of Scie
  • Kim GB; Department of Chemical and Biomolecular Engineering (BK21 Four Program), Metabolic and Biomolecular Engineering National Research Laboratory, Systems Metabolic Engineering and Systems Healthcare Cross-Generation Collaborative Laboratory, Institute for the BioCentury, Korea Advanced Institute of Scie
  • Choi S; Department of Chemical and Biomolecular Engineering (BK21 Four Program), Metabolic and Biomolecular Engineering National Research Laboratory, Systems Metabolic Engineering and Systems Healthcare Cross-Generation Collaborative Laboratory, Institute for the BioCentury, Korea Advanced Institute of Scie
  • Kim JY; Department of Chemical and Biomolecular Engineering (BK21 Four Program), Metabolic and Biomolecular Engineering National Research Laboratory, Systems Metabolic Engineering and Systems Healthcare Cross-Generation Collaborative Laboratory, Institute for the BioCentury, Korea Advanced Institute of Scie
  • Lee SY; Department of Chemical and Biomolecular Engineering (BK21 Four Program), Metabolic and Biomolecular Engineering National Research Laboratory, Systems Metabolic Engineering and Systems Healthcare Cross-Generation Collaborative Laboratory, Institute for the BioCentury, Korea Advanced Institute of Scie
Biotechnol Bioeng ; 120(1): 203-215, 2023 01.
Article em En | MEDLINE | ID: mdl-36128631
Microbial production of various TCA intermediates and related chemicals through the reductive TCA cycle has been of great interest. However, rumen bacteria that naturally possess strong reductive TCA cycle have been rarely studied to produce these chemicals, except for succinic acid, due to their dependence on fumarate reduction to transport electrons for ATP synthesis. In this study, malic acid (MA), a dicarboxylic acid of industrial importance, was selected as a target chemical for mass production using Mannheimia succiniciproducens, a rumen bacterium possessing a strong reductive branch of the TCA cycle. The metabolic pathway was reconstructed by eliminating fumarase to prevent MA conversion to fumarate. The respiration system of M. succiniciproducens was reconstructed by introducing the Actinobacillus succinogenes dimethylsulfoxide (DMSO) reductase to improve cell growth using DMSO as an electron acceptor. Also, the cell membrane was engineered by employing Pseudomonas aeruginosa cis-trans isomerase to enhance MA tolerance. High inoculum fed-batch fermentation of the final engineered strain produced 61 g/L of MA with an overall productivity of 2.27 g/L/h, which is the highest MA productivity reported to date. The systems metabolic engineering strategies reported in this study will be useful for developing anaerobic bioprocesses for the production of various industrially important chemicals.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Mannheimia / Engenharia Metabólica Limite: Animals Idioma: En Ano de publicação: 2023 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Mannheimia / Engenharia Metabólica Limite: Animals Idioma: En Ano de publicação: 2023 Tipo de documento: Article