Nanoscale laser-induced breakdown spectroscopy imaging reveals chemical distribution with subcellular resolution.
Nanoscale Adv
; 2(9): 3983-3990, 2020 Sep 16.
Article
em En
| MEDLINE
| ID: mdl-36132784
ABSTRACT
Understanding chemical compositions is one of the most important parts in exploring the microscopic world. As a simple method for elemental detection, laser-induced breakdown spectroscopy (LIBS) is widely used in materials, geological and life science fields. However, due to the long-existing limitation in spatial resolution, it is difficult for LIBS to play an analytical role in the field of micro-world. Herein, we first report a reliable nanoscale resolution LIBS imaging technique by introducing a sampling laser with a micro-lensed fiber. Through the emission enhancement using the double-pulse laser, we obtained the spectral signal from a sampling crater of less than 500 nanometers in diameter, and visualized the chemical distribution of the self-made grid sample, SIM chip and nano-particles in single cells. The relative limits of detection (RLODs) of In and absolute limits of detection (ALODs) of Al can reach 0.6% and 18.3 fg, respectively.
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MEDLINE
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En
Ano de publicação:
2020
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Article