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Leveraging orthology within maize and Arabidopsis QTL to identify genes affecting natural variation in gravitropism.
Yoshihara, Takeshi; Miller, Nathan D; Rabanal, Fernando A; Myles, Hannah; Kwak, Il-Youp; Broman, Karl W; Sadkhin, Boris; Baxter, Ivan; Dilkes, Brian P; Hudson, Matthew E; Spalding, Edgar P.
Afiliação
  • Yoshihara T; Department of Botany, University of Wisconsin, Madison, WI 53706.
  • Miller ND; Department of Botany, University of Wisconsin, Madison, WI 53706.
  • Rabanal FA; Department of Molecular Biology, Max Planck Institute for Biology Tübingen, 72076 Tübingen, Germany.
  • Myles H; Department of Botany, University of Wisconsin, Madison, WI 53706.
  • Kwak IY; Department of Biostatistics and Medical Informatics, University of Wisconsin, Madison, WI 53706.
  • Broman KW; Department of Biostatistics and Medical Informatics, University of Wisconsin, Madison, WI 53706.
  • Sadkhin B; Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, IL 61801.
  • Baxter I; Donald Danforth Plant Science Center, St. Louis, MO 63132.
  • Dilkes BP; Department of Biochemistry, Purdue University, West Lafayette, IN 47907.
  • Hudson ME; Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, IL 61801.
  • Spalding EP; Department of Crop Sciences, University of Illinois at Urbana-Champaign, Urbana, IL 61801.
Proc Natl Acad Sci U S A ; 119(40): e2212199119, 2022 10 04.
Article em En | MEDLINE | ID: mdl-36161933
Plants typically orient their organs with respect to the Earth's gravity field by a dynamic process called gravitropism. To discover conserved genetic elements affecting seedling root gravitropism, we measured the process in a set of Zea mays (maize) recombinant inbred lines with machine vision and compared the results with those obtained in a similar study of Arabidopsis thaliana. Each of the several quantitative trait loci that we mapped in both species spanned many hundreds of genes, too many to test individually for causality. We reasoned that orthologous genes may be responsible for natural variation in monocot and dicot root gravitropism. If so, pairs of orthologous genes affecting gravitropism may be present within the maize and Arabidopsis QTL intervals. A reciprocal comparison of sequences within the QTL intervals identified seven pairs of such one-to-one orthologs. Analysis of knockout mutants demonstrated a role in gravitropism for four of the seven: CCT2 functions in phosphatidylcholine biosynthesis, ATG5 functions in membrane remodeling during autophagy, UGP2 produces the substrate for cellulose and callose polymer extension, and FAMA is a transcription factor. Automated phenotyping enabled this discovery of four naturally varying components of a conserved process (gravitropism) by making it feasible to conduct the same large-scale experiment in two species.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Arabidopsis / Gravitropismo Idioma: En Ano de publicação: 2022 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Arabidopsis / Gravitropismo Idioma: En Ano de publicação: 2022 Tipo de documento: Article