Your browser doesn't support javascript.
loading
Myostatin in idiopathic inflammatory myopathies: Serum assessment and disease activity.
Mahoudeau, Alexandrine; Anquetil, Céline; Tawara, Nozomu; Khademian, Hossein; Amelin, Damien; Bolko, Loïs; Silvestro, Marco; Cin, Julian Dal; Tendrel, Bérénice; Tardif, Virginie; Mariampillai, Kubéraka; Butler-Browne, Gillian; Benveniste, Olivier; Allenbach, Yves.
Afiliação
  • Mahoudeau A; INSERM, Center of Research in Myology UMRS 974, Sorbonne Université, Paris, France.
  • Anquetil C; INSERM, Center of Research in Myology UMRS 974, Sorbonne Université, Paris, France.
  • Tawara N; Department of Internal Medicine and Clinical Immunology, Sorbonne Université, Pitié-Salpêtrière Hospital, Paris, France.
  • Khademian H; INSERM, Center of Research in Myology UMRS 974, Sorbonne Université, Paris, France.
  • Amelin D; INSERM, Center of Research in Myology UMRS 974, Sorbonne Université, Paris, France.
  • Bolko L; INSERM, Association Institut de Myologie, Center of Research in Myology UMRS 974, Sorbonne Université, Paris, France.
  • Silvestro M; Department of Internal Medicine and Clinical Immunology, Sorbonne Université, Pitié-Salpêtrière Hospital, Paris, France.
  • Cin JD; Department of Internal Medicine and Clinical Immunology, Sorbonne Université, Pitié-Salpêtrière Hospital, Paris, France.
  • Tendrel B; INSERM, Center of Research in Myology UMRS 974, Sorbonne Université, Paris, France.
  • Tardif V; INSERM, Center of Research in Myology UMRS 974, Sorbonne Université, Paris, France.
  • Mariampillai K; INSERM, Center of Research in Myology UMRS 974, Sorbonne Université, Paris, France.
  • Butler-Browne G; Department of Internal Medicine and Clinical Immunology, Sorbonne Université, Pitié-Salpêtrière Hospital, Paris, France.
  • Benveniste O; INSERM, Association Institut de Myologie, Center of Research in Myology UMRS 974, Sorbonne Université, Paris, France.
  • Allenbach Y; INSERM, Center of Research in Myology UMRS 974, Sorbonne Université, Paris, France.
Neuropathol Appl Neurobiol ; 49(1): e12849, 2023 Feb.
Article em En | MEDLINE | ID: mdl-36168256
ABSTRACT

AIMS:

In idiopathic inflammatory myopathies (IIM), disease activity is difficult to assess, and IIM may induce severe muscle damage, especially in immune-mediated necrotising myopathies (IMNM) and inclusion body myositis (IBM). We hypothesise that myostatin, a negative regulator of muscle mass, could be a new biomarker of disease activity and/or muscle damage.

METHODS:

Prospective assessment of myostatin protein level in 447 IIM serum samples (dermatomyositis [DM], n = 157; IBM, n = 72; IMNM, n = 125; and antisynthetase syndrome [ASyS], n = 93) and 59 healthy donors (HD) was performed by ELISA. A gene transcript analysis was also carried out on 18 IIM muscle biopsies and six controls to analyse myostatin and myostatin pathway-related gene expression.

RESULTS:

IIM patients had lower myostatin circulating protein levels and gene expression compared to HD (2379 [1490; 3678] pg/ml vs 4281 [3169; 5787] pg/ml; p < 0.0001 and log2FC = -1.83; p = 0.0005, respectively). Myostatin-related gene expression varied accordingly. Based on the Physician Global Assessment, inactive IIM patients showed higher myostatin levels than active ones. This was the case for all IIM subgroups, except IMNM where low myostatin levels were maintained (2186 [1235; 3815] vs 2349 [1518; 3922] pg/ml; p = 0.4).

CONCLUSIONS:

Myostatin protein and RNA levels are decreased in all IIM patients, and protein levels correlate with disease activity. Inactive ASyS and DM patients have higher myostatin levels than active patients. Myostatin could be a marker of disease activity in these subgroups. However, IMNM patients do not have significant increase in myostatin levels after disease remission. This may highlight a new pathological disease mechanism in IMNM patients.
Assuntos
Palavras-chave

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Miosite de Corpos de Inclusão / Dermatomiosite / Miosite Limite: Humans Idioma: En Ano de publicação: 2023 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Miosite de Corpos de Inclusão / Dermatomiosite / Miosite Limite: Humans Idioma: En Ano de publicação: 2023 Tipo de documento: Article