Visualization of a Limonene Synthesis Metabolon Inside Living Bacteria by Hyperspectral SRS Microscopy.
Adv Sci (Weinh)
; 9(32): e2203887, 2022 11.
Article
em En
| MEDLINE
| ID: mdl-36169112
ABSTRACT
Monitoring biosynthesis activity at single-cell level is key to metabolic engineering but is still difficult to achieve in a label-free manner. Using hyperspectral stimulated Raman scattering imaging in the 670-900 cm-1 region, localized limonene synthesis are visualized inside engineered Escherichia coli. The colocalization of limonene and GFP-fused limonene synthase is confirmed by co-registered stimulated Raman scattering and two-photon fluorescence images. The finding suggests a limonene synthesis metabolon with a polar distribution inside the cells. This finding expands the knowledge of de novo limonene biosynthesis in engineered bacteria and highlights the potential of SRS chemical imaging in metabolic engineering research.
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Base de dados:
MEDLINE
Assunto principal:
Análise Espectral Raman
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Microscopia
Idioma:
En
Ano de publicação:
2022
Tipo de documento:
Article