A Peptide-Based Ligand-Directed Chemistry Enables Protein Functionalization.
Org Lett
; 24(39): 7205-7209, 2022 10 07.
Article
em En
| MEDLINE
| ID: mdl-36169233
ABSTRACT
The ligand-directed (LD) chemistry provides powerful tools for site-specific modification of proteins. We utilized a peptide with an appended methionine (Met) as a ligand; then, the Met thioether was modified into sulfonium which enabled a proximity induced group transfer onto protein cysteine in the vicinity upon peptide-target binding. The sulfonium warhead could be easily constructed with unprotected peptides, and the transferable group scope was conducted on model protein PDZ and its ligand peptides. In addition, a living cell labeling was successfully achieved.
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Base de dados:
MEDLINE
Assunto principal:
Peptídeos
/
Cisteína
Idioma:
En
Ano de publicação:
2022
Tipo de documento:
Article