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Life cycle and functional genomics of the unicellular red alga Galdieria for elucidating algal and plant evolution and industrial use.
Hirooka, Shunsuke; Itabashi, Takeshi; Ichinose, Takako M; Onuma, Ryo; Fujiwara, Takayuki; Yamashita, Shota; Jong, Lin Wei; Tomita, Reiko; Iwane, Atsuko H; Miyagishima, Shin-Ya.
Afiliação
  • Hirooka S; Department of Gene Function and Phenomics, National Institute of Genetics, Shizuoka 411-8540, Japan.
  • Itabashi T; Center for Biosystems Dynamics Research, Laboratory for Cell Field Structure, RIKEN, Hiroshima 739-0046, Japan.
  • Ichinose TM; Center for Biosystems Dynamics Research, Laboratory for Cell Field Structure, RIKEN, Hiroshima 739-0046, Japan.
  • Onuma R; Department of Gene Function and Phenomics, National Institute of Genetics, Shizuoka 411-8540, Japan.
  • Fujiwara T; Department of Gene Function and Phenomics, National Institute of Genetics, Shizuoka 411-8540, Japan.
  • Yamashita S; Department of Genetics, Graduate University for Advanced Studies (SOKENDAI), Shizuoka 411-8540, Japan.
  • Jong LW; Department of Gene Function and Phenomics, National Institute of Genetics, Shizuoka 411-8540, Japan.
  • Tomita R; Department of Gene Function and Phenomics, National Institute of Genetics, Shizuoka 411-8540, Japan.
  • Iwane AH; Department of Genetics, Graduate University for Advanced Studies (SOKENDAI), Shizuoka 411-8540, Japan.
  • Miyagishima SY; Department of Gene Function and Phenomics, National Institute of Genetics, Shizuoka 411-8540, Japan.
Proc Natl Acad Sci U S A ; 119(41): e2210665119, 2022 10 11.
Article em En | MEDLINE | ID: mdl-36194630
Sexual reproduction is widespread in eukaryotes; however, only asexual reproduction has been observed in unicellular red algae, including Galdieria, which branched early in Archaeplastida. Galdieria possesses a small genome; it is polyextremophile, grows either photoautotrophically, mixotrophically, or heterotrophically, and is being developed as an industrial source of vitamins and pigments because of its high biomass productivity. Here, we show that Galdieria exhibits a sexual life cycle, alternating between cell-walled diploid and cell wall-less haploid, and that both phases can proliferate asexually. The haploid can move over surfaces and undergo self-diploidization or generate heterozygous diploids through mating. Further, we prepared the whole genome and a comparative transcriptome dataset between the diploid and haploid and developed genetic tools for the stable gene expression, gene disruption, and selectable marker recycling system using the cell wall-less haploid. The BELL/KNOX and MADS-box transcription factors, which function in haploid-to-diploid transition and development in plants, are specifically expressed in the haploid and diploid, respectively, and are involved in the haploid-to-diploid transition in Galdieria, providing information on the missing link of the sexual life cycle evolution in Archaeplastida. Four actin genes are differently involved in motility of the haploid and cytokinesis in the diploid, both of which are myosin independent and likely reflect ancestral roles of actin. We have also generated photosynthesis-deficient mutants, such as blue-colored cells, which were depleted in chlorophyll and carotenoids, for industrial pigment production. These features of Galdieria facilitate the understanding of the evolution of algae and plants and the industrial use of microalgae.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Actinas / Rodófitas Limite: Animals Idioma: En Ano de publicação: 2022 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Actinas / Rodófitas Limite: Animals Idioma: En Ano de publicação: 2022 Tipo de documento: Article