Methodological and Biological Factors Influencing Global DNA Methylation Results Measured by LINE-1 Pyrosequencing Assay in Colorectal Tissue and Liquid Biopsy Samples.

Szigeti, Krisztina A; Barták, Barbara K; Nagy, Zsófia B; Zsigrai, Sára; Papp, Márton; Márkus, Eszter; Igaz, Peter; Takács, István; Molnár, Béla; Kalmár, Alexandra

Szigeti, Krisztina A; Barták, Barbara K; Nagy, Zsófia B; Zsigrai, Sára; Papp, Márton; Márkus, Eszter; Igaz, Peter; Takács, István; Molnár, Béla; Kalmár, Alexandra.

Afiliação

Szigeti KA; Department of Internal Medicine and Oncology, Faculty of Medicine, Semmelweis University, 1083 Budapest, Hungary.
Barták BK; Department of Internal Medicine and Oncology, Faculty of Medicine, Semmelweis University, 1083 Budapest, Hungary.
Nagy ZB; Department of Internal Medicine and Oncology, Faculty of Medicine, Semmelweis University, 1083 Budapest, Hungary.
Zsigrai S; Department of Internal Medicine and Oncology, Faculty of Medicine, Semmelweis University, 1083 Budapest, Hungary.
Papp M; Centre for Bioinformatics, University of Veterinary Medicine Budapest, 1078 Budapest, Hungary.
Márkus E; Department of Anaesthesia and Intensive Care, Pest County Flor Ferenc Hospital, 2143 Kistarcsa, Hungary.
Igaz P; Department of Internal Medicine and Oncology, Faculty of Medicine, Semmelweis University, 1083 Budapest, Hungary.
Takács I; MTA-SE Molecular Medicine Research Group, Eötvös Loránd Research Network, 1083 Budapest, Hungary.
Molnár B; Department of Endocrinology, Faculty of Medicine, Semmelweis University, 1083 Budapest, Hungary.
Kalmár A; Department of Internal Medicine and Oncology, Faculty of Medicine, Semmelweis University, 1083 Budapest, Hungary.

Int J Mol Sci ; 23(19)2022 Oct 01.

Article em En | MEDLINE | ID: mdl-36232908

Assuntos

Ácidos Nucleicos Livres; Neoplasias Colorretais; Idoso; Fatores Biológicos; Biópsia; Ácidos Nucleicos Livres/genética; Neoplasias Colorretais/genética; Neoplasias Colorretais/patologia; DNA/genética; Metilação de DNA; Feminino; Formaldeído; Sequenciamento de Nucleotídeos em Larga Escala; Humanos; Biópsia Líquida; Elementos Nucleotídeos Longos e Dispersos/genética; Masculino; Sulfitos

Palavras-chave

LINE-1 bisulfite pyrosequencing; colorectal cancer; global DNA methylation; long interspersed nuclear element-1

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Neoplasias Colorretais / Ácidos Nucleicos Livres Limite: Aged / Female / Humans / Male Idioma: En Ano de publicação: 2022 Tipo de documento: Article

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