Quantification of cytosol and membrane proteins in rumen epithelium of sheep with low or high CH4 emission phenotype.
PLoS One
; 17(10): e0273184, 2022.
Article
em En
| MEDLINE
| ID: mdl-36256644
ABSTRACT
BACKGROUND:
Ruminant livestock are a major contributor to Australian agricultural sector carbon emissions. Variation in methane (CH4) produced from enteric microbial fermentation of feed in the reticulo-rumen of sheep differs with different digestive functions.METHOD:
We isolated rumen epithelium enzymatically to extract membrane and cytosol proteins from sheep with high (H) and low (L) CH4 emission. Protein abundance was quantified using SWATH-mass spectrometry.RESULTS:
The research found differences related to the metabolism of glucose, lactate and processes of cell defence against microbes in sheep from each phenotype. Enzymes in the methylglyoxal pathway, a side path of glycolysis, resulting in D-lactate production, differed in abundance. In the H CH4 rumen epithelium the enzyme hydroxyacylglutathione hydrolase (HAGH) was 2.56 fold higher in abundance, whereas in the L CH4 epithelium lactate dehydrogenase D (LDHD) was 1.93 fold higher. Malic enzyme 1 which converts D-lactate to pyruvate via the tricarboxylic cycle was 1.57 fold higher in the L CH4 phenotype. Other proteins that are known to regulate cell defence against microbes had differential abundance in the epithelium of each phenotype.CONCLUSION:
Differences in the abundance of enzymes involved in the metabolism of glucose were associated with H and L CH4 phenotype sheep. Potentially this represents an opportunity to use protein markers in the rumen epithelium to select low CH4 emitting sheep.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Rúmen
/
Proteínas de Membrana
Limite:
Animals
País como assunto:
Oceania
Idioma:
En
Ano de publicação:
2022
Tipo de documento:
Article