MicroRNA-9a-5p-NOX4 inhibits intestinal inflammatory injury by regulating the M1 polarization of intestinal macrophages.
J Biochem Mol Toxicol
; 37(2): e23245, 2023 Feb.
Article
em En
| MEDLINE
| ID: mdl-36281492
ABSTRACT
We found that the expression of microRNA (miRNA)-9a-5p decreased in inflammatory bowel diseases (IBD; ulcerative colitis and Crohn's disease). Further, we revealed the effects and mechanisms of miRNA-9a-5p for regulating IBD progression. In C57BL/6N mice, IBD was induced with dextran sodium sulfate (DSS), and the effects of endogenous miRNA-9a-5p were mimicked/antagonized through intraperitoneal injection of miRNA-9a-5p agomir and antagomir. In animal experimentation, agomir could inhibit intestinal inflammation and tissue damage, and reduce the mucosal barrier permeability. Antagomir, on the other hand, could promote barrier damage, whose effect was associated with the M1 macrophage polarization. This study finds that miRNA-9a-5p targets NOX4 to suppress ROS production, which plays an important role in mucosal barrier damage in IBD.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Doenças Inflamatórias Intestinais
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Colite
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MicroRNAs
Limite:
Animals
Idioma:
En
Ano de publicação:
2023
Tipo de documento:
Article