Your browser doesn't support javascript.
loading
Prevalence and temporal dynamics of Cryptosporidium spp., Giardia duodenalis, and Blastocystis sp. among toddlers attending day-care centres in Spain. A prospective molecular-based longitudinal study.
Hernández-Castro, Carolina; Dashti, Alejandro; Vusirikala, Amoolya; Balasegaram, Sooria; Köster, Pamela Carolina; Bailo, Begoña; Imaña, Elena; López, Andrea; Llorente, María Teresa; González-Barrio, David; Sánchez, Sergio; Carmena, David.
Afiliação
  • Hernández-Castro C; Parasitology Reference and Research Laboratory, National Centre for Microbiology, 28220, Majadahonda, Madrid, Spain.
  • Dashti A; Parasitology Group, Faculty of Medicine, Academic Corporation for the Study of Tropical Pathologies, University of Antioquia, Medellín, Colombia.
  • Vusirikala A; Parasitology Reference and Research Laboratory, National Centre for Microbiology, 28220, Majadahonda, Madrid, Spain.
  • Balasegaram S; UK Field Epidemiology Training Programme, UK Health Security Agency, London, SE1 8UG, UK.
  • Köster PC; UK Health Security Agency, London, SE1 8UG, UK.
  • Bailo B; Parasitology Reference and Research Laboratory, National Centre for Microbiology, 28220, Majadahonda, Madrid, Spain.
  • Imaña E; Parasitology Reference and Research Laboratory, National Centre for Microbiology, 28220, Majadahonda, Madrid, Spain.
  • López A; Parasitology Reference and Research Laboratory, National Centre for Microbiology, 28220, Majadahonda, Madrid, Spain.
  • Llorente MT; Food and Waterborne Bacterial Infections Reference and Research Laboratory, National Centre for Microbiology, 28220, Majadahonda, Madrid, Spain.
  • González-Barrio D; Food and Waterborne Bacterial Infections Reference and Research Laboratory, National Centre for Microbiology, 28220, Majadahonda, Madrid, Spain.
  • Sánchez S; Parasitology Reference and Research Laboratory, National Centre for Microbiology, 28220, Majadahonda, Madrid, Spain.
  • Carmena D; Food and Waterborne Bacterial Infections Reference and Research Laboratory, National Centre for Microbiology, 28220, Majadahonda, Madrid, Spain. sergio.sanchez@isciii.es.
Eur J Pediatr ; 182(1): 213-223, 2023 Jan.
Article em En | MEDLINE | ID: mdl-36282323
Giardia duodenalis, Cryptosporidium spp., and Blastocystis sp. are common intestinal eukaryotic parasites affecting children in developed and resource-limited countries. Lack of information on the epidemiology and long-term stability in asymptomatic children complicates interpretation of transmission and pathogenesis. To assess the occurrence, genetic diversity, and temporal dynamics of intestinal eukaryotic parasites in young children, 679 stool samples from 125 toddlers attending six public day-care centres in Central Spain were collected bimonthly within a 1-year period. Detection and identification of species/genotypes were based on PCR and Sanger sequencing methods. Four eukaryotic species were identified: G. duodenalis (2.5‒31.6%), Cryptosporidium spp. (0.0‒2.4%), Blastocystis sp. (2.5‒6.4%), and Entamoeba dispar (0.0‒0.9%). Entamoeba histolytica and Enterocytozoon bieneusi were undetected. Sequence analyses identified assemblage A (63.6%) and B (36.4%) within G. duodenalis (n = 11), C. hominis (40%), C. parvum (40%), and C. wrairi (20%) within Cryptosporidium spp. (n = 5), and ST1 (3.8%), ST2 (46.2%), ST3 (15.4%), and ST4 (34.6%) within Blastocystis sp. (n = 26). Giardia duodenalis sub-assemblage AII/AIII was detected in a toddler for 10 consecutive months. Stable carriage of Blastocystis ST2 allele 9, ST3 allele 34, and ST4 allele 42 was demonstrated in five toddlers for up to 1 year.   Conclusions: Giardia duodenalis and Blastocystis sp. were common in toddlers attending day-care centres in Central Spain. Long-term infection/colonization periods by the same genetic variant were observed for G. duodenalis (up to 10 months) and Blastocystis sp. (up to 12 months). What is Known: • Asymptomatic carriage of G. duodenalis and Blastocystis sp. is frequent in toddlers. • The epidemiology and long-term stability of these eukaryotes in asymptomatic young children is poorly understood. What is New: • Long-term colonization/infection periods by the same genetic variant were described for Blastocystis sp. (up to 12 months) and G. duodenalis (up to 10 months).
Assuntos
Palavras-chave

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Giardíase / Giardia lamblia / Blastocystis / Criptosporidiose / Cryptosporidium / Enteropatias Parasitárias Tipo de estudo: Observational_studies / Prevalence_studies / Risk_factors_studies Limite: Child, preschool / Humans País como assunto: Europa Idioma: En Ano de publicação: 2023 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Giardíase / Giardia lamblia / Blastocystis / Criptosporidiose / Cryptosporidium / Enteropatias Parasitárias Tipo de estudo: Observational_studies / Prevalence_studies / Risk_factors_studies Limite: Child, preschool / Humans País como assunto: Europa Idioma: En Ano de publicação: 2023 Tipo de documento: Article