Intravascular Administration of Acridine Orange and Zoledronate in a Bone Metastasis Model of Breast Cancer.
Anticancer Res
; 42(11): 5357-5363, 2022 Nov.
Article
em En
| MEDLINE
| ID: mdl-36288846
ABSTRACT
BACKGROUND/AIM:
This study evaluated the effect of haematogenous administration of acridine orange (AO) alone and in combination with zoledronate (ZOL) on bone metastases. MATERIALS ANDMETHODS:
E0771 cells (1.0×105 cells/10 µl) were injected directly into the right femur of female mice. The mice were divided into five groups according to treatment (drugs and irradiation) and were reared and sacrificed after 6 weeks. Micro-computed tomography (µCT) was performed to calculate the destruction rate of the femur bone. We measured tumour weight and volume at sacrifice and performed terminal deoxynucleotidyl transferase dUTP Nick-End Labelling staining of tumours.RESULTS:
At 4 weeks, the bone destruction rate was lower in the AO+ZOL group than in the radiation group. At 6 weeks, the AO+ZOL group had a lower bone destruction rate than the control and radiation groups; the ZOL group had a lower rate than the radiation group. The AO and AO+ZOL groups had suppressed tumour weight and volume compared to the control and radiation groups. The number of extraosseous apoptotic cells was higher in the AO+ZOL group than in all other groups except the AO group.CONCLUSION:
In a model of local bone metastasis of breast cancer, haematogenous administration of AO reduced tumour size and more so when combined with ZOL.Palavras-chave
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Osteólise
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Neoplasias Ósseas
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Neoplasias da Mama
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Conservadores da Densidade Óssea
Limite:
Animals
Idioma:
En
Ano de publicação:
2022
Tipo de documento:
Article