Characterization of drug effects on cell cultures from phase-contrast microscopy images.
Comput Biol Med
; 151(Pt A): 106171, 2022 12.
Article
em En
| MEDLINE
| ID: mdl-36306582
ABSTRACT
In this work, we classify chemotherapeutic agents (topoisomerase inhibitors) based on their effect on U-2 OS cells. We use phase-contrast microscopy images, which are faster and easier to obtain than fluorescence images and support live cell imaging. We use a convolutional neural network (CNN) trained end-to-end directly on the input images without requiring for manual segmentations or any other auxiliary data. Our method can distinguish between tested cytotoxic drugs with an accuracy of 98%, provided that their mechanism of action differs, outperforming previous work. The results are even better when substance-specific concentrations are used. We show the benefit of sharing the extracted features over all classes (drugs). Finally, a 2D visualization of these features reveals clusters, which correspond well to known class labels, suggesting the possible use of our methodology for drug discovery application in analyzing new, unseen drugs.
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Base de dados:
MEDLINE
Assunto principal:
Redes Neurais de Computação
/
Técnicas de Cultura de Células
Idioma:
En
Ano de publicação:
2022
Tipo de documento:
Article