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Nebulized fusion inhibitory peptide protects cynomolgus macaques from measles virus infection.
Reynard, Olivier; Gonzalez, Claudia; Dumont, Claire; Iampietro, Mathieu; Ferren, Marion; Le Guellec, Sandrine; Laurie, Lajoie; Mathieu, Cyrille; Carpentier, Gabrielle; Roseau, Georges; Bovier, Francesca T; Zhu, Yun; Le Pennec, Deborah; Montharu, Jérome; Addetia, Amin; Greninger, Alexander L; Alabi, Christopher A; Brisebard, Elise; Moscona, Anne; Vecellio, Laurent; Porotto, Matteo; Horvat, Branka.
Afiliação
  • Reynard O; CIRI, Centre International de Recherche en Infectiologie, INSERM U1111, CNRS, UMR5308, Univ Lyon, Université Claude Bernard Lyon 1, École Normale Supérieure de Lyon, 21 Avenue Tony Garnier, 69007, Lyon, France.
  • Gonzalez C; CIRI, Centre International de Recherche en Infectiologie, INSERM U1111, CNRS, UMR5308, Univ Lyon, Université Claude Bernard Lyon 1, École Normale Supérieure de Lyon, 21 Avenue Tony Garnier, 69007, Lyon, France.
  • Dumont C; CIRI, Centre International de Recherche en Infectiologie, INSERM U1111, CNRS, UMR5308, Univ Lyon, Université Claude Bernard Lyon 1, École Normale Supérieure de Lyon, 21 Avenue Tony Garnier, 69007, Lyon, France.
  • Iampietro M; CIRI, Centre International de Recherche en Infectiologie, INSERM U1111, CNRS, UMR5308, Univ Lyon, Université Claude Bernard Lyon 1, École Normale Supérieure de Lyon, 21 Avenue Tony Garnier, 69007, Lyon, France.
  • Ferren M; CIRI, Centre International de Recherche en Infectiologie, INSERM U1111, CNRS, UMR5308, Univ Lyon, Université Claude Bernard Lyon 1, École Normale Supérieure de Lyon, 21 Avenue Tony Garnier, 69007, Lyon, France.
  • Le Guellec S; DTF-Aerodrug, R&D aerosolltherapy department of DTF medical (Saint Etienne, France), Faculté de médecine, Université de Tours, 37032, Tours, France.
  • Laurie L; Université de Tours, Institut national de recherche pour l'agriculture, l'alimentation et l'environnement (INRAe), UMR1282, Infectiologie et santé publique (ISP), Tours, France.
  • Mathieu C; CIRI, Centre International de Recherche en Infectiologie, INSERM U1111, CNRS, UMR5308, Univ Lyon, Université Claude Bernard Lyon 1, École Normale Supérieure de Lyon, 21 Avenue Tony Garnier, 69007, Lyon, France.
  • Carpentier G; PST-A, Université de Tours, Tours, France.
  • Roseau G; PST-A, Université de Tours, Tours, France.
  • Bovier FT; Center for Host-Pathogen Interaction, Department of Pediatrics, Columbia University Vagelos College of Physicians and Surgeons, New York, NY, USA.
  • Zhu Y; Center for Host-Pathogen Interaction, Department of Pediatrics, Columbia University Vagelos College of Physicians and Surgeons, New York, NY, USA.
  • Le Pennec D; Laboratory of Infection and Virology, Beijing Children's Hospital, Capital Medical University, National Center for Children's Health, Beijing, 100045, China.
  • Montharu J; INSERM, Research Center for Respiratory Diseases, CEPR U1100, Université de Tours, 37032, Tours, France.
  • Addetia A; PST-A, Université de Tours, Tours, France.
  • Greninger AL; Department of Laboratory Medicine and Pathology, University of Washington Medical Center, Seattle, WA, USA.
  • Alabi CA; Department of Laboratory Medicine and Pathology, University of Washington Medical Center, Seattle, WA, USA.
  • Brisebard E; Robert Frederick Smith School of Chemical and Biomolecular Engineering, Cornell University, Ithaca, NY, USA.
  • Moscona A; UMR703, PAnTher APEX, INRAE/Oniris, Nantes, France.
  • Vecellio L; Center for Host-Pathogen Interaction, Department of Pediatrics, Columbia University Vagelos College of Physicians and Surgeons, New York, NY, USA.
  • Porotto M; Department of Microbiology and Immunology, Columbia University Vagelos College of Physicians & Surgeons, New York, NY, USA.
  • Horvat B; Department of Physiology & Cellular Biophysics, Columbia University Vagelos College of Physicians & Surgeons, New York, NY, USA.
Nat Commun ; 13(1): 6439, 2022 10 28.
Article em En | MEDLINE | ID: mdl-36307480
Measles is the most contagious airborne viral infection and the leading cause of child death among vaccine-preventable diseases. We show here that aerosolized lipopeptide fusion inhibitor, derived from heptad-repeat regions of the measles virus (MeV) fusion protein, blocks respiratory MeV infection in a non-human primate model, the cynomolgus macaque. We use a custom-designed mesh nebulizer to ensure efficient aerosol delivery of peptide to the respiratory tract and demonstrate the absence of adverse effects and lung pathology in macaques. The nebulized peptide efficiently prevents MeV infection, resulting in the complete absence of MeV RNA, MeV-infected cells, and MeV-specific humoral responses in treated animals. This strategy provides an additional means to fight against respiratory infection in non-vaccinated people, that can be readily translated to human trials. It presents a proof-of-concept for the aerosol delivery of fusion inhibitory peptides to protect against measles and other airborne viruses, including SARS-CoV-2, in case of high-risk exposure.
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: COVID-19 / Sarampo Limite: Animals / Humans Idioma: En Ano de publicação: 2022 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: COVID-19 / Sarampo Limite: Animals / Humans Idioma: En Ano de publicação: 2022 Tipo de documento: Article