Using FRET-Based Biosensor Cells to Study the Seeding Activity of Tau and α-Synuclein.
Methods Mol Biol
; 2551: 125-145, 2023.
Article
em En
| MEDLINE
| ID: mdl-36310201
ABSTRACT
Two fluorescence resonance energy transfer (FRET)-based biosensor cell lines developed several years ago by the Diamond group (University of Texas, Southwestern) have allowed convenient, sensitive, and specific measurement of the intracellular aggregation of tau and α-synuclein following the addition of oligomer or small-aggregate "seeds" of these proteins from various sources, and an advancement relative to similar single-fluorophore systems. These biosensor cell lines allow researchers to both visualize the intracellular aggregates of tau or α-synuclein and measure intracellular aggregation with high sensitivity using a FRET signal in flow cytometry. Here we provide detailed protocols for generating seeds, culturing the biosensor cells, measuring intracellular aggregates by flow cytometry, and analyzing the results and discuss the utility of the technique with the aim of characterizing factors involved in the regulation of intracellular tau and α-synuclein aggregation.
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Base de dados:
MEDLINE
Assunto principal:
Técnicas Biossensoriais
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Alfa-Sinucleína
Idioma:
En
Ano de publicação:
2023
Tipo de documento:
Article