Micro RNA-411 Expression Improves Cardiac Phenotype Following Myocardial Infarction in Mice.
JACC Basic Transl Sci
; 7(9): 859-875, 2022 Sep.
Article
em En
| MEDLINE
| ID: mdl-36317138
ABSTRACT
Induction of endogenous regenerative capacity has emerged as one promising approach to repair damaged hearts following myocardial infarction (MI). Re-expression of factors that are exclusively expressed during embryonic development may reactivate the ability of adult cardiomyocytes to regenerate. Here, we identified miR-411 as a potent inducer of cardiomyocyte proliferation. Overexpression of miR-411 in the heart significantly increased cardiomyocyte proliferation and survival in a model MI. We found that miR-411 enhances the activity of YAP, the main downstream effector of the Hippo pathway, in cardiomyocytes. In conclusion, miR-411 induces cardiomyocyte regeneration and improves cardiac function post-MI likely by modulating the Hippo/YAP pathway.
CVEC, cardiac vascular endothelial cells; EdU, 5-ethynyl-2'-deoxyuridine; Hippo pathway; LAD, left anterior descending coronary artery; MI, myocardial infarction; MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide; NFAT, nuclear factor of activated T cells; NRCF, neonatal rat cardiac fibroblast; NRCM, neonatal rat cardiomyocytes; PCR, polymerase chain reaction; PEI, polyethylenimine; cTnI, cardiac troponin I; cardiac remodeling; heart failure; miRNA, microRNA; microRNA-411; myocardial infarction; pHH3, phosphohistone H3; qPCR, quantitative PCR
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Base de dados:
MEDLINE
Tipo de estudo:
Prognostic_studies
Idioma:
En
Ano de publicação:
2022
Tipo de documento:
Article