[Clinical significance of serum miRNA-146, OX-LDL and ROS expression in patients with primary ovarian insufficiency].
Zhonghua Yi Xue Yi Chuan Xue Za Zhi
; 39(11): 1211-1215, 2022 Nov 10.
Article
em Zh
| MEDLINE
| ID: mdl-36317205
ABSTRACT
OBJECTIVE:
To investigate the clinical significance of miRNA-146, OX-LDL and ROS in patients with primary ovarian insufficiency (POI).METHODS:
100 patients with POI were prospectively collected and 100 women with normal ovarian function were randomly selected as control group. Serum miRNA-146 expression level was detected by qRT-PCR and serum OX-LDL and ROS expression levels were detected by ELISA. Ovarian granulosa cells of mouse were transfected with miRNA-146 mimics or inhibitors, and then treated with OX-LDL. Cell viability, colony forming ability, apoptosis rate and toll like receptor 4 (TLR4)/nuclear factor-κB (NF-κB) of pathway proteins were evaluated respectively.RESULTS:
Compared with control group, the expression level of miRNA-146 in POI group was significantly lower, the expression level of OX-LDL and ROS were significantly higher, and the ovarian volume and peak systolic blood flow velocity of ovarian artery were significantly decreased in POI group. Upregulation of miRNA-146 expression had a protective effect on OX-LDL injured ovarian granulosa cells, as evidenced by increased ovarian granulosa cell viability and colony number, reduced apoptosis, and downregulation of TLR4/NF-κB expression.CONCLUSION:
miRNA-146 can target downstream TLR4/NF-κB signaling pathway affects oxidative stress and inflammatory response of POI induced by OX-LDL and ROS, and is expected to become a biomarker for early prediction of POI and a new target for treatment.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Insuficiência Ovariana Primária
/
MicroRNAs
Tipo de estudo:
Prognostic_studies
Limite:
Animals
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Female
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Humans
Idioma:
Zh
Ano de publicação:
2022
Tipo de documento:
Article