Study of spatiotemporal regulation of kinase signaling using genetically encodable molecular tools.
Curr Opin Chem Biol
; 71: 102224, 2022 12.
Article
em En
| MEDLINE
| ID: mdl-36347198
ABSTRACT
Precise spatiotemporal organization and regulation of signal transduction networks are essential for cellular response to internal and external cues. To understand how this biochemical activity architecture impacts cellular function, many genetically encodable tools which regulate kinase activity at a subcellular level have been developed. In this review, we highlight various types of genetically encodable molecular tools, including tools to regulate endogenous kinase activity and biorthogonal techniques to perturb kinase activity. Finally, we emphasize the use of these tools alongside biosensors for kinase activity to measure and perturb kinase activity in real time for a better understanding of the cellular biochemical activity architecture.
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Base de dados:
MEDLINE
Assunto principal:
Fosfotransferases
/
Técnicas Biossensoriais
Idioma:
En
Ano de publicação:
2022
Tipo de documento:
Article