Distinguishing Gastrointestinal Leiomyomas From Muscularis Propria in Biopsy Specimens by Differential Expression of S100 Immunohistochemical Stain.
Am J Clin Pathol
; 159(1): 53-59, 2023 01 04.
Article
em En
| MEDLINE
| ID: mdl-36367375
ABSTRACT
OBJECTIVES:
Interpreting small biopsy specimens or fine-needle aspirations of gastrointestinal tract (GI) smooth muscle lesions may be challenging when the differential diagnosis includes leiomyoma vs muscularis propria (MP). We evaluated the utility of S100 staining in distinguishing GI leiomyomas from MP.METHODS:
A search was conducted in our laboratory information system for cases of leiomyomas arising within the GI tract (2004-2021). Site-matched controls containing MP were selected (2018-2020). Five high-power fields (hpf) were counted on S100 immunohistochemical stains by two pathologists in the resections and by three different blinded pathologists in the biopsy specimens and analyzed.RESULTS:
The median S100 count was 2.5/5 hpf in leiomyoma resection cases (nâ =â 38), which was significantly lower than the median count of 548/5 hpf in MP (nâ =â 19) with a P value ofâ <.0001. The median S100 count in biopsy specimens (nâ =â 16) was 1.2/5 hpf and within the expected range of 1 to 104/5 hpf (minimum-maximum value) established by the leiomyoma resections. S100 counts in the normal MP were significantly higher than those observed in leiomyomas (Pâ <â .001).CONCLUSIONS:
S100 staining can aid in distinguishing a leiomyoma from MP in the GI tract, which is especially helpful when evaluating cases with limited sampling.Palavras-chave
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Base de dados:
MEDLINE
Assunto principal:
Corantes
/
Leiomioma
Limite:
Humans
Idioma:
En
Ano de publicação:
2023
Tipo de documento:
Article