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Orthogonal protein decoration of DNA nanostructures based on SpyCatcher-SpyTag interaction.
Kröll, Sandra; Schneider, Leonie; Wadhwani, Parvesh; Rabe, Kersten S; Niemeyer, Christof M.
Afiliação
  • Kröll S; Institute for Biological Interfaces (IBG 1), Karlsruhe Institute of Technology (KIT), Hermann-von-Helmholtz-Platz 1, 76344, Karlsruhe, Germany. niemeyer@kit.edu.
  • Schneider L; Institute for Biological Interfaces (IBG 1), Karlsruhe Institute of Technology (KIT), Hermann-von-Helmholtz-Platz 1, 76344, Karlsruhe, Germany. niemeyer@kit.edu.
  • Wadhwani P; Department of Molecular Biophysics (IBG 2), Karlsruhe Institute of Technology (KIT), Hermann-von-Helmholtz-Platz 1, 76344, Karlsruhe, Germany.
  • Rabe KS; Institute for Biological Interfaces (IBG 1), Karlsruhe Institute of Technology (KIT), Hermann-von-Helmholtz-Platz 1, 76344, Karlsruhe, Germany. niemeyer@kit.edu.
  • Niemeyer CM; Institute for Biological Interfaces (IBG 1), Karlsruhe Institute of Technology (KIT), Hermann-von-Helmholtz-Platz 1, 76344, Karlsruhe, Germany. niemeyer@kit.edu.
Chem Commun (Camb) ; 58(97): 13471-13474, 2022 Dec 06.
Article em En | MEDLINE | ID: mdl-36383063
We present an efficient and readily applicable strategy for the covalent ligation of proteins to DNA origami by using the SpyCatcher-SpyTag (SC-ST) connector system. This approach showed orthogonality with other covalent connectors and has been used exemplarily for the immobilization and study of stereoselective ketoreductases to gain insight into the spatial arrangement of enzymes on DNA nanostructures.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: DNA / Nanoestruturas / Proteínas de Ligação a DNA Idioma: En Ano de publicação: 2022 Tipo de documento: Article
Texto completo
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PubMed Links
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: DNA / Nanoestruturas / Proteínas de Ligação a DNA Idioma: En Ano de publicação: 2022 Tipo de documento: Article