m6A RNA methylation impairs gene expression variability and reproductive thermotolerance in Arabidopsis.
Genome Biol
; 23(1): 244, 2022 11 23.
Article
em En
| MEDLINE
| ID: mdl-36419179
ABSTRACT
Heat-imposed crop failure is often attributed to reduced thermotolerance of floral tissues; however, the underlying mechanism remains unknown. Here, we demonstrate that m6A RNA methylation increases in Arabidopsis flowers and negatively regulates gene expression variability. Stochastic gene expression provides flexibility to cope with environmental stresses. We find that reduced transcriptional fluctuation is associated with compromised activation of heat-responsive genes. Moreover, disruption of an RNA demethylase AtALKBH10B leads to lower gene expression variability, suppression of heat-activated genes, and strong reduction of plant fertility. Our work proposes a novel role for RNA methylation in the bet-hedging strategy of heat stress response.
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MEDLINE
Assunto principal:
Arabidopsis
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Proteínas de Arabidopsis
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Termotolerância
Idioma:
En
Ano de publicação:
2022
Tipo de documento:
Article