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Ultra-high-performance liquid chromatography using a fused-core particle column for fast analysis of propolis phenolic compounds.
Contieri, Letícia S; de Souza Mesquita, Leonardo M; Sanches, Vitor L; Viganó, Juliane; Kamikawachi, Renan Canute; Vilegas, Wagner; Rostagno, Mauricio A.
Afiliação
  • Contieri LS; Multidisciplinary Laboratory of Food and Health (LabMAS), School of applied sciences (FCA), University of Campinas (UNICAMP), Limeira, Brazil.
  • de Souza Mesquita LM; Multidisciplinary Laboratory of Food and Health (LabMAS), School of applied sciences (FCA), University of Campinas (UNICAMP), Limeira, Brazil.
  • Sanches VL; Multidisciplinary Laboratory of Food and Health (LabMAS), School of applied sciences (FCA), University of Campinas (UNICAMP), Limeira, Brazil.
  • Viganó J; Centro de Ciências da Natureza, Universidade Federal de São Carlos, Rod. Lauri Simões de Barros, Buri, Brazil.
  • Kamikawachi RC; UNESP - São Paulo State University, Institute of Biosciences, São Vicente, Brazil.
  • Vilegas W; UNESP - São Paulo State University, Institute of Biosciences, São Vicente, Brazil.
  • Rostagno MA; Multidisciplinary Laboratory of Food and Health (LabMAS), School of applied sciences (FCA), University of Campinas (UNICAMP), Limeira, Brazil.
J Sep Sci ; 46(3): e2200440, 2023 Feb.
Article em En | MEDLINE | ID: mdl-36449264
Propolis is a bee product with a complex chemical composition formed by several species from different geographical origins. The complex propolis composition requires an accurate and reproducible characterization of samples to standardize the quality of the material sold to consumers. This work developed an ultra-high-performance liquid chromatography with a photodiode array detector method to analyze propolis phenolic compounds based on the two key propolis biomarkers, Artepillin C and p-Coumaric acid. This choice was made due to the complexity of the sample with the presence of several compounds. The optimized method was hyphenated with mass spectrometry detection allowing the detection of 23 different compounds. A step-by-step strategy was used to optimize temperature, flow rate, mobile phase composition, and re-equilibration time. Reverse-phase separation was achieved with a C18 fused-core column packed with the commercially available smallest particles (1.3 nm). Using a fused-core column with ultra-high-performance liquid chromatography allows highly efficient, sensitive, accurate, and reproducible determination of compounds extracted from propolis with an outstanding sample throughput and resolution. Optimized conditions permitted the separation of the compounds in 5.50 min with a total analysis time (sample-to-sample) of 6.50 min.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Própole Idioma: En Ano de publicação: 2023 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Própole Idioma: En Ano de publicação: 2023 Tipo de documento: Article