3D models; AM, Ameloblastoma; BPE, Bovine pituitary extract; BSA, Bovine serum albumin; CCN, Cellular communication network factor 2; DMEM, Dulbecco's modified Eagle medium; ECM, Extracellular matrix; FBS, Foetal bovine serum; Fibroblasts; H&E, Haematoxylin and eosin; HFF-2, Human fibroblasts (HFF-2); HGF, Primary gingival fibroblasts; IF, Immunofluorescence; IHC; IHC, Immunohistochemistry; M, Molar; MEM, Minimal Essential Medium; MIQE, Minimum Information for Publication of Quantitative Real-Time PCR Experiments; MMPs, Matrix metalloproteinases; MRC5, Human lung fibroblasts; Min, Minutes; N.A, Neutralising agent; PTHLH, Parathyroid Hormone Like Hormone; RANK, The tumour necrosis factor (TNF) superfamily members receptor activator of nuclear factor kappa-B receptor; RANKL; RANKL, The tumour necrosis factor (TNF) superfamily members receptor activator of nuclear factor kappa-B ligand (TNFSF11); RT, Room temperature; SD, Standard deviation; SEM, Standard error mean; SPARC, anti-osteonectin; TGF-ß, Transforming growth factor; TME, Tumour microenvironment; TNF, Tumour necrosis factor; Tumour microenvironment; dl-CGH, Double-layered collagen gel hemisphere; hOB, Human osteoblasts; α-SMA, alpha-smooth muscle actin