Large Protein Assemblies for High-Relaxivity Contrast Agents: The Case of Gadolinium-Labeled Asparaginase.
Bioconjug Chem
; 33(12): 2411-2419, 2022 12 21.
Article
em En
| MEDLINE
| ID: mdl-36458591
ABSTRACT
Biologics are emerging as the most important class of drugs and are used to treat a large variety of pathologies. Most of biologics are proteins administered in large amounts, either by intramuscular injection or by intravenous infusion. Asparaginase is a large tetrameric protein assembly, currently used against acute lymphoblastic leukemia. Here, a gadolinium(III)-DOTA derivative has been conjugated to asparaginase, and its relaxation properties have been investigated to assess its efficiency as a possible theranostic agent. The field-dependent 1H longitudinal relaxation measurements of water solutions of gadolinium(III)-labeled asparaginase indicate a very large increase in the relaxivity of this paramagnetic protein complex with respect to small gadolinium chelates, opening up the possibility of its use as an MRI contrast agent.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Asparaginase
/
Meios de Contraste
Idioma:
En
Ano de publicação:
2022
Tipo de documento:
Article