Genetic analysis of genome sequence characteristics of two lumpy skin disease viruses isolated from China.
BMC Vet Res
; 18(1): 426, 2022 Dec 07.
Article
em En
| MEDLINE
| ID: mdl-36476204
ABSTRACT
BACKGROUND:
Lumpy skin disease (LSD) is an acute or subacute infectious disease caused by lumpy skin disease virus (LSDV) of genus Capripoxvirus. The outbreaks of LSD were confirmed in the Yili area of the Xinjiang autonomous region in August 2019 and the Fujian province in June 2020. We detected LSDV in our daily monitoring work, then isolated, identified and sequenced the virus, and analyzed the whole genome characteristics of the isolated strain.RESULTS:
Whole genome sequencing revealed that the strains isolated were all LSDV and were named as LSDV XJ201901 and LSDV FJ2019. The results showed that the identity based on whole genome sequences between LSDV XJ201901 and LSDV FJ2019 was 100% and the identity based on whole genome sequences between the two isolated strains and the global LSDV strains was 97.28%-99.99%, with the strain LSDV72/PrachuapKhiriKhan/Thailand/2021 (99.99%) having the highest sequence identity. Analysis of potential recombination events revealed that a total of 18 potential recombination events were identified in strains LSDV XJ201901 and LSDV FJ2019. The two strains are a recombination of Neethling vaccine LW 1959 (GeneBank AF409138.1) with KSGP 0240 (GeneBank KX683219.1). It was observed that Neethling vaccine LW 1959 (11/18) and KSGP 0240 (10/18) are involved in most of the potential recombination events.CONCLUSIONS:
The virus isolate in this study was LSDV and was identified as a vaccine recombinant strain. The most likely potential parent strains of the two strains in this study are Neethling vaccine LW 1959 and KSGP 0240. The strains in this study are very similar to those isolated in East and Southeast Asia since 2019.Palavras-chave
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Base de dados:
MEDLINE
Assunto principal:
Vacinas
/
Doenças dos Bovinos
/
Doença Nodular Cutânea
/
Vírus da Doença Nodular Cutânea
Tipo de estudo:
Prognostic_studies
Limite:
Animals
País como assunto:
Asia
Idioma:
En
Ano de publicação:
2022
Tipo de documento:
Article