[Effects of Different Treatment Methods on the Contents of Related Growth Factors Released by Platelet Rich Plasma].
Zhongguo Shi Yan Xue Ye Xue Za Zhi
; 30(6): 1834-1838, 2022 Dec.
Article
em Zh
| MEDLINE
| ID: mdl-36476912
ABSTRACT
OBJECTIVE:
To evaluate the effect of sonication, repeated freeze-thaw cycles, calcium salt solution and their combination on the content of related growth factors (GFs) released by platelet rich plasma (PRP).METHODS:
Twenty PRPs from healthy blood donors were divided into 9 groups, including sonication group, freeze-thaw group, calcium gluconate group, calcium chloride group, sonication + calcium gluconate group, sonication + calcium chloride group, freeze-thaw + calcium gluconate group, freeze-thaw + calcium chloride group, and sonication + freeze-thaw group. After PRP activated by above 9 methods, the content of transforming growth factor-ß1 (TGF-ß1), vascular endothelial growth factor (VEGF), and platelet-derived growth factor-BB (PDGF-BB) were detected by ELISA.RESULTS:
The platelet concentration of the samples was (966.7±202.6)×109/L. The content of TGF-ß1 in sonication + freeze-thaw group was the highest, while the lowest was in freeze-thaw group. The content of VEGF in freeze-thaw + calcium chloride group was the highest, while the lowest was in calcium gluconate group. The content of PDGF-BB in sonication + freeze-thaw group was the highest, while the lowest was in calcium gluconate group. There was no significant differences in the three GFs between calcium gluconate group and calcium chloride group.CONCLUSION:
Among the 9 activated methods of PRP, there is no difference between two calcium salt solutions. And the combination of repeated freeze-thaw cycles and sonication may be the best treatment method to promote PRP to release GFs, while calcium gluconate is the weakest way.Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Plasma Rico em Plaquetas
/
Fator de Crescimento Transformador beta1
Limite:
Humans
Idioma:
Zh
Ano de publicação:
2022
Tipo de documento:
Article