Sensitive immunoassay of Legionella using multivalent conjugates of engineered VHHs.

Kiyose, Norihiko; Miyazaki, Nobuo; Furuhata, Katsunori; Ito, Yuji

Kiyose, Norihiko; Miyazaki, Nobuo; Furuhata, Katsunori; Ito, Yuji.

Afiliação

Kiyose N; Graduate School of Science and Engineering, Kagoshima University, 1-21-35 Korimoto, Kagoshima 890-0065, Japan.
Miyazaki N; ARK Resource Co., Ltd., 383-2, Nakahara-machi, Nishi-ku, Kumamoto 861-5271, Japan.
Furuhata K; ARK Resource Co., Ltd., 383-2, Nakahara-machi, Nishi-ku, Kumamoto 861-5271, Japan.
Ito Y; School of Life and Environmental Science, Azabu University, 1-17-71 Fuchinobe, Chuo-ku, Sagamihara-shi, Kanagawa 252-5201, Japan.

J Biochem ; 173(3): 185-195, 2023 Mar 07.

Article em En | MEDLINE | ID: mdl-36525357

Assuntos

Legionella; Doença dos Legionários; Anticorpos de Domínio Único; Humanos; Antígenos; Imunoensaio; Cadeias Pesadas de Imunoglobulinas

Palavras-chave

VHH.Abbreviations: Abs, antibodies; BSA, bovine serum albumin; CDR, complementarity determining region; CFU, colony forming unit; DBCO, dibenzylcyclooctyne; ELISA, enzyme-linked immunosorbent assay; FR, framework region; HcAbs, heavy chain antibodies; KLH, keyhole limpet hemocyanin; LpSG1, Legionella pneumophila serogroup1; MALDI-TOFMS, matrix assisted laser desorption/ionization time of flight mass spectrometry; NHS, N-hydroxysuccinimide; PBMC, peripheral blood mononuclear cells; PCR, polymerase chain reaction; RT-PCR, reverse transcription PCR; SDS-PAGE, sodium do-decyl sulphate-polyacrylamide gel electrophoresis; TMB, 3,3',5,5'-tetramethylbenzidine solution; alpaca; antibody; engineering; immunoassay

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Legionella / Doença dos Legionários / Anticorpos de Domínio Único Tipo de estudo: Diagnostic_studies Limite: Humans Idioma: En Ano de publicação: 2023 Tipo de documento: Article

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