Fabrication of a novel electrochemical biosensor based on a molecular imprinted polymer-aptamer hybrid receptor for lysozyme determination.
Anal Bioanal Chem
; 415(5): 899-911, 2023 Feb.
Article
em En
| MEDLINE
| ID: mdl-36544030
ABSTRACT
In this work, a novel, sensitive, and rapid electrochemical biosensor was employed to detect lysozyme (Lys) using a double receptor of molecular imprinted polymer (MIP)-aptamer. First, a glassy carbon electrode (GCE) was modified with a nanocomposite consisting of multi-wall carbon nanotubes (MWCNTs), nitrogen-doped carbon quantum dots (N-CQDs), and chitosan. Subsequently, aptamer (Apt)-Lys complex was immobilized on MWCNTs-N-CQDs-chitosan/GCE via binding between carboxyl groups present in the nanocomposite and the terminal amine groups of the aptamer. Following that, methylene blue monomer was electrochemically polymerized around the Apt-Lys complex on the MWCNTs-N-CQDs-chitosan/GCE surface. Finally, after the template removal, the remaining cavities along with the aptamers created a new hybrid receptor of MIP-aptamer. The MWCNTs-N-CQDs-chitosan nanocomposite could provide large amounts of carboxyl groups for binding to amino-functionalized aptamers, considerable electrical conductivity, and a high surface-to-volume ratio. These beneficial features facilitated the Apt-Lys complex immobilization and gave improved electrochemical signal. The obtained MIP-aptamer hybrid receptor allowed lysozyme determination even at concentrations as low as 4.26 fM within the functional range of 1 fM to 100 nM.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Técnicas Biossensoriais
/
Nanotubos de Carbono
/
Quitosana
/
Aptâmeros de Nucleotídeos
Idioma:
En
Ano de publicação:
2023
Tipo de documento:
Article