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Analyzing the Tumor-Immune Microenvironment by Flow Cytometry.
Santinon, François; Young, Yoon Kow; Del Rincón, Sonia V; Mann, Koren K.
Afiliação
  • Santinon F; Lady Davis Institute for Medical Research, McGill University, Montreal, QC, Canada. francoissantinon@gmail.com.
  • Young YK; Lady Davis Institute for Medical Research, McGill University, Montreal, QC, Canada.
  • Del Rincón SV; Lady Davis Institute for Medical Research, McGill University, Montreal, QC, Canada.
  • Mann KK; Gerald Bronfman Department of Oncology, McGill University, Montreal, QC, Canada.
Methods Mol Biol ; 2614: 17-36, 2023.
Article em En | MEDLINE | ID: mdl-36587116
Flow cytometry is an essential tool for studying the tumor-immune microenvironment. It allows us to quickly quantify and identify multiple cell types in a heterogeneous sample. This chapter provides an overview of the flow cytometry instrumentation and a discussion of the appropriate considerations and steps in building a reproducible flow cytometry staining panel. We present an updated lymphoid tissue and solid tumor-infiltrating leucocyte flow cytometry staining protocol and an example of flow cytometry data analysis.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Neoplasias Limite: Humans Idioma: En Ano de publicação: 2023 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Neoplasias Limite: Humans Idioma: En Ano de publicação: 2023 Tipo de documento: Article