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Activity and stability of lipase from Candida Antarctica after treatment in pressurized fluids.
Feiten, Mirian Cristina; Morigi, Iasmin; Di Luccio, Marco; Oliveira, José Vladimir.
Afiliação
  • Feiten MC; Department of Technology, State University of Maringá (UEM), Angelo Moreira da Fonseca Ave, Umuarama, Paraná, 87506-370, Brazil. mirianfeiten.mf@gmail.com.
  • Morigi I; Department of Chemical and Food Engineering, Federal University of Santa Catarina (UFSC), Technology Center/C.P. 476, Florianópolis, Santa Catarina, 88040-900, Brazil.
  • Di Luccio M; Department of Chemical and Food Engineering, Federal University of Santa Catarina (UFSC), Technology Center/C.P. 476, Florianópolis, Santa Catarina, 88040-900, Brazil.
  • Oliveira JV; Department of Chemical and Food Engineering, Federal University of Santa Catarina (UFSC), Technology Center/C.P. 476, Florianópolis, Santa Catarina, 88040-900, Brazil.
Biotechnol Lett ; 45(2): 287-298, 2023 Feb.
Article em En | MEDLINE | ID: mdl-36592260
Lipase B from Candida antarctica (CalB) is one of the biocatalysts most used in organic synthesis due to its ability to act in several medium, wide substrate specificity and enantioselectivity, tolerance to non-aqueous environment, and resistance to thermal deactivation. Thus, the objective of this work was to treat CalB in supercritical carbon dioxide (SC-CO2) and liquefied petroleum gas (LPG), and measure its activity before and after high-pressure treatment. Residual specific hydrolytic activities of 132% and 142% were observed when CalB was exposed to SC-CO2 at 35 â„ƒ, 75 bar and 1 h and to LPG at 65 â„ƒ, 30 bar and 1 h, respectively. Residual activity of the enzyme treated at high pressure was still above 100% until the 20th day of storage at low temperatures. There was no difference on the residual activity loss of CalB treated with LPG and stored at different temperatures over time. Greater difference was observed between CalB treated with CO2 and flash-frozen in liquid nitrogen (- 196 â„ƒ) followed by storage in freezer (- 10 â„ƒ) and CalB stored in freezer at - 10 â„ƒ. Such findings encourage deeper studies on CalB as well as other enzymes behavior under different types of pressurized fluids aiming at industrial application.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Enzimas Imobilizadas / Lipase Idioma: En Ano de publicação: 2023 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Enzimas Imobilizadas / Lipase Idioma: En Ano de publicação: 2023 Tipo de documento: Article