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Antibiotic perseverance increases the risk of resistance development.
Brandis, Gerrit; Larsson, Jimmy; Elf, Johan.
Afiliação
  • Brandis G; Department of Cell and Molecular Biology, Science for Life Laboratory, Uppsala University 751 24, Uppsala, Sweden.
  • Larsson J; Department of Cell and Molecular Biology, Science for Life Laboratory, Uppsala University 751 24, Uppsala, Sweden.
  • Elf J; Department of Cell and Molecular Biology, Science for Life Laboratory, Uppsala University 751 24, Uppsala, Sweden.
Proc Natl Acad Sci U S A ; 120(2): e2216216120, 2023 01 10.
Article em En | MEDLINE | ID: mdl-36595701
The rise of antibiotic-resistant bacterial infections poses a global threat. Antibiotic resistance development is generally studied in batch cultures which conceals the heterogeneity in cellular responses. Using single-cell imaging, we studied the growth response of Escherichia coli to sub-inhibitory and inhibitory concentrations of nine antibiotics. We found that the heterogeneity in growth increases more than what is expected from growth rate reduction for three out of the nine antibiotics tested. For two antibiotics (rifampicin and nitrofurantoin), we found that sub-populations were able to maintain growth at lethal antibiotic concentrations for up to 10 generations. This perseverance of growth increased the population size and led to an up to 40-fold increase in the frequency of antibiotic resistance mutations in gram-negative and gram-positive species. We conclude that antibiotic perseverance is a common phenomenon that has the potential to impact antibiotic resistance development across pathogenic bacteria.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Escherichia coli / Antibacterianos Tipo de estudo: Etiology_studies / Risk_factors_studies Idioma: En Ano de publicação: 2023 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Escherichia coli / Antibacterianos Tipo de estudo: Etiology_studies / Risk_factors_studies Idioma: En Ano de publicação: 2023 Tipo de documento: Article