Isolation and phenotypic characterization of human and nonhuman primate intestinal lamina propria mononuclear cells.
STAR Protoc
; 3(4): 101815, 2022 12 16.
Article
em En
| MEDLINE
| ID: mdl-36595919
ABSTRACT
Isolation of viable immune cells from tissues is critically important to characterize cellular and molecular processes during homeostasis and disease. Here, we provide an optimized protocol to achieve high yields of viable intestinal lamina propria mononuclear cells (LPMCs). We describe steps for intestinal tissue collection from humans and nonhuman primates, followed by mechanical disruption and enzymatic digestion. Furthermore, we detail characterization of the mononuclear phagocyte (MP) subtypes by flow cytometry analysis. The protocol is repeatable and scalable for downstream applications. For complete details on the use and execution of this protocol, please refer to Cavarelli et al. (2022).
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Mucosa Intestinal
/
Leucócitos
Limite:
Animals
/
Humans
Idioma:
En
Ano de publicação:
2022
Tipo de documento:
Article