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Breastfeeding enrichment of B. longum subsp. infantis mitigates the effect of antibiotics on the microbiota and childhood asthma risk.
Dai, Darlene L Y; Petersen, Charisse; Hoskinson, Courtney; Del Bel, Kate L; Becker, Allan B; Moraes, Theo J; Mandhane, Piushkumar J; Finlay, B Brett; Simons, Elinor; Kozyrskyj, Anita L; Patrick, David M; Subbarao, Padmaja; Bode, Lars; Azad, Meghan B; Turvey, Stuart E.
Afiliação
  • Dai DLY; Department of Pediatrics, BC Children's Hospital, University of British Columbia, 950 West 28th Avenue, Vancouver, BC V6H 3V4, Canada.
  • Petersen C; Department of Pediatrics, BC Children's Hospital, University of British Columbia, 950 West 28th Avenue, Vancouver, BC V6H 3V4, Canada.
  • Hoskinson C; Department of Pediatrics, BC Children's Hospital, University of British Columbia, 950 West 28th Avenue, Vancouver, BC V6H 3V4, Canada; Department of Microbiology and Immunology, University of British Columbia, Vancouver, BC V6T 1Z3, Canada.
  • Del Bel KL; Department of Pediatrics, BC Children's Hospital, University of British Columbia, 950 West 28th Avenue, Vancouver, BC V6H 3V4, Canada.
  • Becker AB; Section of Allergy and Immunology, University of Manitoba, Winnipeg, MB R3E 3P5, Canada; Department of Pediatrics and Child Health, University of Manitoba, Winnipeg, MB R3A 1S1, Canada.
  • Moraes TJ; Department of Pediatrics, The Hospital for Sick Children, Toronto, ON M5G 1X8, Canada.
  • Mandhane PJ; Department of Pediatrics, University of Alberta, Edmonton, AB T6G 1C9, Canada.
  • Finlay BB; Department of Microbiology and Immunology, University of British Columbia, Vancouver, BC V6T 1Z3, Canada; Michael Smith Laboratories, University of British Columbia, Vancouver, BC V6T 1Z4, Canada; Department of Biochemistry and Molecular Biology, University of British Columbia, Vancouver, BC V6T 1Z3
  • Simons E; Section of Allergy and Immunology, University of Manitoba, Winnipeg, MB R3E 3P5, Canada; Department of Pediatrics and Child Health, University of Manitoba, Winnipeg, MB R3A 1S1, Canada.
  • Kozyrskyj AL; Department of Pediatrics, University of Alberta, Edmonton, AB T6G 1C9, Canada.
  • Patrick DM; School of Population and Public Health, University of British Columbia, Vancouver, BC V6T 1Z3, Canada; British Columbia Centre for Disease Control, Vancouver, BC V5Z 4R4, Canada.
  • Subbarao P; Department of Pediatrics, The Hospital for Sick Children, Toronto, ON M5G 1X8, Canada; Department of Medicine, McMaster University, Hamilton, ON L8N 3Z5, Canada; Dalla Lana School of Public Health, University of Toronto, Toronto, ON M5T 3M7, Canada.
  • Bode L; Department of Pediatrics, University of California, San Diego, La Jolla, CA 92161, USA; Larsson-Rosenquist Foundation Mother-Milk-Infant Center of Research Excellence, University of California, San Diego, La Jolla, CA 92093, USA.
  • Azad MB; Department of Pediatrics and Child Health, University of Manitoba, Winnipeg, MB R3A 1S1, Canada; Department of Food and Human Nutritional Sciences, University of Manitoba, Winnipeg, MB R3T 2N2, Canada; Manitoba Interdisciplinary Lactation Centre (MILC), Children's Hospital Research Institute of Mani
  • Turvey SE; Department of Pediatrics, BC Children's Hospital, University of British Columbia, 950 West 28th Avenue, Vancouver, BC V6H 3V4, Canada. Electronic address: sturvey@cw.bc.ca.
Med ; 4(2): 92-112.e5, 2023 02 10.
Article em En | MEDLINE | ID: mdl-36603585
BACKGROUND: Early antibiotic exposure is linked to persistent disruption of the infant gut microbiome and subsequent elevated pediatric asthma risk. Breastfeeding acts as a primary modulator of the gut microbiome during early life, but its effect on asthma development has remained unclear. METHODS: We harnessed the CHILD cohort to interrogate the influence of breastfeeding on antibiotic-associated asthma risk in a subset of children (n = 2,521). We then profiled the infant microbiomes in a subset of these children (n = 1,338) using shotgun metagenomic sequencing and compared human milk oligosaccharide and fatty acid composition from paired maternal human milk samples for 561 of these infants. FINDINGS: Children who took antibiotics without breastfeeding had 3-fold higher asthma odds, whereas there was no such association in children who received antibiotics while breastfeeding. This benefit was associated with widespread "re-balancing" of taxonomic and functional components of the infant microbiome. Functional changes associated with asthma protection were linked to enriched Bifidobacterium longum subsp. infantis colonization. Network analysis identified a selection of fucosylated human milk oligosaccharides in paired maternal samples that were positively associated with B. infantis and these broader functional changes. CONCLUSIONS: Our data suggest that breastfeeding and antibiotics have opposing effects on the infant microbiome and that breastfeeding enrichment of B. infantis is associated with reduced antibiotic-associated asthma risk. FUNDING: This work was supported in part by the Canadian Institutes of Health Research; the Allergy, Genes and Environment Network of Centres of Excellence; Genome Canada; and Genome British Columbia.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Asma / Sulfaleno / Microbiota Tipo de estudo: Etiology_studies / Prognostic_studies / Risk_factors_studies Limite: Child / Female / Humans / Infant País como assunto: America do norte Idioma: En Ano de publicação: 2023 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Asma / Sulfaleno / Microbiota Tipo de estudo: Etiology_studies / Prognostic_studies / Risk_factors_studies Limite: Child / Female / Humans / Infant País como assunto: America do norte Idioma: En Ano de publicação: 2023 Tipo de documento: Article