Solid-phase capture and profiling of open chromatin by spatial ATAC.
Nat Biotechnol
; 41(8): 1085-1088, 2023 08.
Article
em En
| MEDLINE
| ID: mdl-36604544
ABSTRACT
Current methods for epigenomic profiling are limited in their ability to obtain genome-wide information with spatial resolution. We introduce spatial ATAC, a method that integrates transposase-accessible chromatin profiling in tissue sections with barcoded solid-phase capture to perform spatially resolved epigenomics. We show that spatial ATAC enables the discovery of the regulatory programs underlying spatial gene expression during mouse organogenesis, lineage differentiation and in human pathology.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Cromatina
/
Transposases
Limite:
Animals
/
Humans
Idioma:
En
Ano de publicação:
2023
Tipo de documento:
Article