Bump-and-hole engineering of human polypeptide N-acetylgalactosamine transferases to dissect their protein substrates and glycosylation sites in cells.

Calle, Beatriz; Gonzalez-Rodriguez, Edgar; Mahoney, Keira E; Cioce, Anna; Bineva-Todd, Ganka; Tastan, Omur Y; Roustan, Chloe; Flynn, Helen; Malaker, Stacy A; Schumann, Benjamin

Calle, Beatriz; Gonzalez-Rodriguez, Edgar; Mahoney, Keira E; Cioce, Anna; Bineva-Todd, Ganka; Tastan, Omur Y; Roustan, Chloe; Flynn, Helen; Malaker, Stacy A; Schumann, Benjamin.

Afiliação

Calle B; Department of Chemistry, Imperial College London, London W12 0BZ, UK; Chemical Glycobiology Laboratory, The Francis Crick Institute, London NW1 1AT, UK; Tumour-Host Interaction Laboratory, The Francis Crick Institute, London NW1 1AT, UK.
Gonzalez-Rodriguez E; Department of Chemistry, Imperial College London, London W12 0BZ, UK; Chemical Glycobiology Laboratory, The Francis Crick Institute, London NW1 1AT, UK.
Mahoney KE; Department of Chemistry, Yale University, New Haven, CT 06511, USA.
Cioce A; Department of Chemistry, Imperial College London, London W12 0BZ, UK; Chemical Glycobiology Laboratory, The Francis Crick Institute, London NW1 1AT, UK.
Bineva-Todd G; Chemical Glycobiology Laboratory, The Francis Crick Institute, London NW1 1AT, UK.
Tastan OY; Chemical Glycobiology Laboratory, The Francis Crick Institute, London NW1 1AT, UK.
Roustan C; Structural Biology Science Technology Platform, The Francis Crick Institute, London NW1 1AT, UK.
Flynn H; Proteomics Science Technology Platform, The Francis Crick Institute, London NW1 1AT, UK.
Malaker SA; Department of Chemistry, Yale University, New Haven, CT 06511, USA.
Schumann B; Department of Chemistry, Imperial College London, London W12 0BZ, UK; Chemical Glycobiology Laboratory, The Francis Crick Institute, London NW1 1AT, UK. Electronic address: b.schumann@imperial.ac.uk.

STAR Protoc ; 4(1): 101974, 2023 03 17.

Article em En | MEDLINE | ID: mdl-36633947

Assuntos

N-Acetilgalactosaminiltransferases; Proteínas; Humanos; Glicosilação; Proteínas/metabolismo; Peptídeos/química; Polissacarídeos/química; N-Acetilgalactosaminiltransferases/genética; N-Acetilgalactosaminiltransferases/química; N-Acetilgalactosaminiltransferases/metabolismo

Palavras-chave

Cell-based Assays; Mass Spectrometry; Molecular/Chemical Probes; Protein Biochemistry; Proteomics

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Proteínas / N-Acetilgalactosaminiltransferases Limite: Humans Idioma: En Ano de publicação: 2023 Tipo de documento: Article

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